Open access ESI-TOF/MS and GC/MS for organic chemists

面向有机化学家的开放访问 ESI-TOF/MS 和 GC/MS

• 批准号: 6730744
• 负责人: TODD D WILLIAMS
• 金额: $ 33.32万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730744
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; gas chromatography mass spectrometry

DESCRIPTION (provided by applicant): The University of Kansas Mass Spectrometry Laboratory (MSL) proposes to build an open access mass spectrometry system capable of supporting research in the synthesis of organic compounds. Users would be NIH-funded researchers primarily in the departments of Chemistry, Medicinal Chemistry and Pharmaceutical Chemistry. Support is requested for the purchase of two systems, a GC/MS and an ESITOF instrument, each with automated sample introduction. The ionization methods would be El or CI on a GC/MS instrument with a quadrupole analyzer and electrospray with a Time of Flight analyzer. The intention is to make available a set of instruments that an investigator could easily use by presenting a sample in an auto-injector vial, logging in, selecting a "basic" description of an experiment then launching the spectral acquisition. The result would be a spectrum, automatically printed, presenting the major ions detected from the sample. Time from sample introduction to spectra is minutes. Spectra will be saved electronically for later review and a workstation will be available to re-analyze files. There are 18 active synthetic groups in Chemistry, Medicinal Chemistry and Pharmaceutical Chemistry. Seven of the most active will present a broad structural diversity among samples ranging from low molecular weight intermediates through reasonably complex drugs (mw 600-1100) to coordination compounds, synthetic peptides and the occasional modified protein. The instruments will be managed by the permanent staff of a successful core facility. The primary motivations to create this capability are four fold: 1) increase sample through-put capacity without adding staff; 2) decrease turnaround time with automatic sample introduction; 3) lower per analysis cost to investigators and; 4) replace a 23 year old instrument. The addition of new instruments is important to maintain recent sustained growth in the research environment.

描述（由申请人提供）：堪萨斯大学质谱实验室（MSL）提议建立一个开放访问质谱系统，能够支持有机化合物合成的研究。用户将主要是NIH资助的研究人员，主要是化学，药物化学和药物化学部门。请求购买两个系统，一个GC/MS和一个Esitof仪器，每个系统都有自动样本简介。电离方法将是带有四极分析仪的GC/MS仪器上的EL或CI，并带有飞行分析仪的电喷雾。目的是提供一组研究者，可以通过在自动注射器小瓶中展示样品，登录，选择实验的“基本”描述，然后启动光谱采集来轻松使用。结果将是一个自动打印的频谱，从样品中呈现了主要离子。样品介绍到光谱的时间为分钟。光谱将以电子方式保存，以供以后审核，并且将有一个工作站重新分析文件。化学，药物化学和药物化学中有18个活跃的合成基团。从低分子量中间体到合理复杂的药物（MW 600-1100）到配位化合物，合成肽和偶尔修饰蛋白的样品，最活跃的七个将呈现出广泛的结构多样性。这些工具将由成功的核心设施的常任人员管理。创建此功能的主要动机是四倍：1）在不增加员工的情况下增加样本贯穿能力； 2）随着自动样品引入减少周转时间； 3）调查人员的每个分析成本较低； 4）更换23年历史的乐器。新工具的增加对于维持研究环境中持续增长的持续增长很重要。

项目成果

Bioactivity profiling with parallel mass spectrometry reveals an assemblage of green tea metabolites affording protection against human huntingtin and alpha-synuclein toxicity.

使用平行质谱法进行的生物活性分析揭示了绿茶代谢物的组合，可提供针对人类亨廷顿蛋白和 α-突触核蛋白毒性的保护。

• DOI: 10.1021/jf072241x
• 发表时间: 2007
• 期刊: Journal of agricultural and food chemistry
• 影响因子: 6.1
• 作者: Williams,RussellB;Gutekunst,WillR;Joyner,PMatthew;Duan,Wenzhen;Li,Qing;Ross,ChristopherA;Williams,ToddD;Cichewicz,RobertH
• 通讯作者: Cichewicz,RobertH