PEPTIDE SYNTHESIZER AMINO ACID ANALYZER

这项资助计划建立一个肽合成，纯化， 分析系统在堪萨斯大学。 近几年 生物化学系、化学系、药物化学系、微生物学系， 和药物化学已经证明了对 合成肽，以探索酶催化机制， 抗体的产生和纯化，用于检查稳定性 肽和蛋白质药物的运输，并详细说明 神经肽的结构和活性。 为了满足日益增长的需求，我们 建议建立一个肽实验室，作为大学的一部分， 生物化学研究服务实验室，在查尔斯博士的指导下 欺骗。 要求的设备包括：应用生物系统模型 431 A肽合成系统，具有单个反应容器和树脂 取样器、带压力计的半岛4 B型HF反应装置、沃茨 625 LC分析型HPLC、沃茨PicoTag氨基酸分析系统和 沃茨DeltaPrep 3000制备型HPLC。 PI将监督设计， 合成和纯化策略的开发，以及 实验室人员的培训，和博士C Decedue将监督日常 仪器的操作和日常维护。 该设施将 设计用于完整的服务，包括靶肽的设计， 如有必要，或为要求参与研究的研究人员提供部分服务， 合成或纯化阶段。 该中心还将容纳 特殊合成程序，偶联非天然或合成 氨基酸，以及使用t-boc 或F-MOC化学。 这项服务的目标将是提高肽- 在大学相关的生物医学研究，通过提供及时的 以成本回收和纯化产品。

This grant proposes to establish a peptide synthesis, purification, and analysis system at the University of Kansas. In the last few years the Departments of Biochemistry, Chemistry, Medicinal Chemistry, Microbiology, and Pharmaceutical Chemistry have demonstrated an increasing demand for the synthesis of peptides in order to explore enzyme catalytic mechanisms, for the generation and purification of antibodies, for examining the stability and transport of peptide and protein pharmaceuticals, and for detailing the structure and activities of neuropeptides. To meet this growing demand, we propose to establish a peptide laboratory as part of the University's Biochemical Research Service Laboratory, under the direction of Dr. Charles Decedue. The requested equipment include: an Applied Biosystems Model 431A peptide synthesis system with single reaction vessel and resin sampler, a Peninsula Type 4B HF reaction apparatus with manometer, a Waters 625LC analytical HPLC, a Waters PicoTag amino acid analysis system, and a Waters DeltaPrep 3000 preparative HPLC. The PI will oversee the design and development of synthetic and purification strategies, as well as the training of laboratory personnel, and Dr. C Decedue will oversee day to day operations of the instruments and routine maintenance. The facility will be designed for complete service, including the design of target peptides, if necessary, or partial service for researchers requesting involvement in the synthesis or purification stages. The center will also accommodate special synthetic procedures, the coupling of non-natural or synthetic amino acids, and the fragment condensation of peptides using either t-boc or f-moc chemistry. The goal of this service will be to enhance peptide- related biomedical research at the University by providing timely turnaround and a purified product at cost.