PEPTIDE SYNTHESIZER AMINO ACID ANALYZER

这笔赠款建议建立一种多肽的合成、纯化和 堪萨斯大学的分析系统。在过去的几年里 生物化学、化学、药物化学、微生物学、 和药物化学已经显示出对 为了探索酶的催化机制，合成多肽， 抗体的产生和纯化，用于检测稳定性 和多肽和蛋白质药物的运输，并详细说明 神经肽的结构和活性。为了满足不断增长的需求，我们 建议建立一个多肽实验室，作为大学 生物化学研究服务实验室，由查尔斯博士指导 德克杜。所要求的设备包括：应用生物系统模型 用单一反应容器和树脂合成431A肽的系统 带压力计的半岛4B型氢氟化氢反应装置采样器 625LC分析高效液相色谱法、Waters PicoTag氨基酸分析系统和 Waters DeltaPrep 3000制备型高效液相色谱法。私家侦探将监督设计和 合成和提纯策略的发展，以及 实验室人员的培训，C·德克杜博士将负责日常工作 仪器的操作和日常维护。该设施将 为全面服务而设计，包括目标肽的设计， 如有必要，或为要求参与的研究人员提供部分服务 合成或提纯阶段。该中心还将容纳 特殊的合成程序，非天然或合成的耦合 氨基酸，以及使用t-BOC的多肽的片段缩合 或者f-moc化学。这项服务的目标将是增强多肽- 及时为大学提供相关的生物医学研究 扭亏为盈，并以成本价获得纯化产品。

This grant proposes to establish a peptide synthesis, purification, and analysis system at the University of Kansas. In the last few years the Departments of Biochemistry, Chemistry, Medicinal Chemistry, Microbiology, and Pharmaceutical Chemistry have demonstrated an increasing demand for the synthesis of peptides in order to explore enzyme catalytic mechanisms, for the generation and purification of antibodies, for examining the stability and transport of peptide and protein pharmaceuticals, and for detailing the structure and activities of neuropeptides. To meet this growing demand, we propose to establish a peptide laboratory as part of the University's Biochemical Research Service Laboratory, under the direction of Dr. Charles Decedue. The requested equipment include: an Applied Biosystems Model 431A peptide synthesis system with single reaction vessel and resin sampler, a Peninsula Type 4B HF reaction apparatus with manometer, a Waters 625LC analytical HPLC, a Waters PicoTag amino acid analysis system, and a Waters DeltaPrep 3000 preparative HPLC. The PI will oversee the design and development of synthetic and purification strategies, as well as the training of laboratory personnel, and Dr. C Decedue will oversee day to day operations of the instruments and routine maintenance. The facility will be designed for complete service, including the design of target peptides, if necessary, or partial service for researchers requesting involvement in the synthesis or purification stages. The center will also accommodate special synthetic procedures, the coupling of non-natural or synthetic amino acids, and the fragment condensation of peptides using either t-boc or f-moc chemistry. The goal of this service will be to enhance peptide- related biomedical research at the University by providing timely turnaround and a purified product at cost.