CONFOCAL MICROSCOPE AND 3-D GRAPHICS WORKSTATION

共焦显微镜和 3D 图形工作站

• 批准号: 3521205
• 负责人: DAVID C VAN ESSEN
• 金额: $ 13.9万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-21 至 1992-05-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521205
• 关键词: Primates; biomedical equipment resource; brain; computer graphics /printing; computer program /software; computer system design /evaluation; confocal scanning microscopy; cytoskeleton; embryo /fetus; embryogenesis; fluorescent dye /probe; imaging /visualization /scanning; magnetic resonance imaging; molecular dynamics; neurogenesis; noninvasive diagnosis; positron emission tomography; single cell analysis

We request funding for the acquisition of equipment needed for the 3-dimensional (3-D) reconstruction and analysis of biological structures. The major equipment items are a laser-scanning confocal microscope (Zeiss LSM) and a high-performance 3-D graphics workstation (Silicon Graphics IRIS VGX series). These will be part of an integrated effort in biological imaging, which will involve 14 research labs in the Biology Division at Caltech. One set of projects will utilize the confocal system for studying single cells, early embryos, and other structures at the microscopic level. Another set of projects will be aimed at generating 3-D reconstructions of macroscopic structures, including the brains of monkeys, humans, and other species. For many of these projects, we will be able to use commercially available software for image analysis and 3-D reconstruction. We also intend to develop software for specialized applications, but this activity will not be directly supported by the requested funding. To promote and coordinate these efforts, we have established a Biological Imaging Center on campus. This will be a professionally staffed facility, and we anticipate that it will substantially enhance the efficiency, ease, and flexibility with which the requested equipment will be used.

我们要求提供资金，用于购买 生物结构的三维(3-D)重建和分析。 主要设备是激光扫描共聚焦显微镜(蔡司 LSM)和高性能3-D图形工作站(Silicon Graphics Iris VGX系列)。这些将是综合努力的一部分， 生物成像，将涉及14个生物学研究实验室 加州理工大学的分部。一组项目将利用共焦 用于研究单细胞、早期胚胎和其他结构的系统 微观层面。另一组项目将旨在 生成宏观结构的3-D重建，包括 猴子、人类和其他物种的大脑。对于其中的许多 项目，我们将能够使用商用软件来 图像分析和三维重建。我们还打算发展 用于专门应用程序的软件，但此活动不会 由所要求的资金直接支持。推进和协调 在这些努力下，我们在校园里建立了生物影像中心。 这将是一个配备专业人员的设施，我们预计它 将大大提高效率、易用性和灵活性 所请求的设备将被使用的位置。