AUTOMATED DNA SEQUENCING SYSTEM

自动化 DNA 测序系统

• 批准号: 3521105
• 负责人: C THOMAS CASKEY
• 金额: $ 18.2万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-10 至 1992-05-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521105
• 关键词: DNA; Lesch Nyhan syndrome; biomedical automation; biomedical equipment purchase; biomedical facility; chromosomes; cystic fibrosis; developmental neurobiology; gene expression; gene mutation; genetic mapping; genetic transcription; hereditary motor and sensory neuropathy; protein structure function; radiation genetics; regulatory gene

DNA sequence analysis is currently provided at the Institute for Molecular Genetics (IMG) at Baylor College of Medicine by a core facility. The sequencing core provides a mechanism whereby investigators can obtain DNA sequence information without having to dedicate their own laboratory resources. Fragments are provided to the facility in a variety of forms and in different DNA doning vectors, and the data is returned via a computer network. The sequencing capacity of the facility is almost saturated with the current 373A Applied Biosystems automated DNA sequencer operated on every working day and approximately 50% of weekend days. The methods for DNA template preparation and for manipulating the sequencing reaction components are now being simplified, and the demands from individual projects are increasing. We estimate a minimum of a 100% increase in demand for the facilities throughput within 12 months. To cater for this additional capacity an Applied Biosystems 373 Plus DNA Sequencing system is requested. The instrument consists of a robot for generating DNA templates and a fluorescent DNA sequencer to analyze the reaction products. The instrument will more than double the capacity of the DNA sequencing facility as the robot can be used to supply products for both the new and existing fluorescence analyzers. The wide interest in defining and manipulating individual DNA fragments means the increase in rate and capacity for the generation of DNA sequence will benefit all IMG investigators and their staff.

DNA序列分析目前在研究所提供， 分子遗传学（IMG）在贝勒医学院的核心 设施。 测序核心提供了一种机制， 研究人员可以获得DNA序列信息，而不必 投入自己的实验室资源。 片段被提供给 以各种形式和在不同的DNA供体载体中的设施，和 数据通过计算机网络返回。 的测序能力 该设施几乎饱和，目前373A应用 生物系统自动DNA测序仪在每个工作日运行， 约50%的周末 DNA模板的方法 制备和操作测序反应组分的方法是 现在正在简化，个别项目的要求是 增加。 我们估计， 12个月内的设施吞吐量。 为了满足这一额外的 Applied Biosystems 373 Plus DNA测序系统 所请求 该仪器由一个用于生成DNA的机器人组成 模板和荧光DNA测序仪来分析反应 产品. 该仪器将使DNA的容量增加一倍以上 排序设施作为机器人可用于供应产品， 新的和现有的荧光分析仪。 广泛关注 定义和操纵单个DNA片段意味着 DNA序列产生的速度和能力将使所有人受益 IMG调查员及其工作人员。