AUTOMATED DNA SEQUENCING SYSTEM

DNA序列分析目前由该研究所提供 贝勒医学院的分子遗传学 设施。测序核心提供了一种机制，由此 调查人员可以获得DNA序列信息 奉献自己的实验室资源。片段被提供给 以各种形式和不同的DNA给予载体提供便利，以及 数据通过计算机网络返回。的测序能力 工厂几乎饱和了目前应用的373A BiosSystems自动DNA测序仪在每个工作日运行， 大约50%的周末。DNA模板的几种方法 用于操作测序反应组分的制备 现在正在简化，来自个别项目的需求是 越来越多。我们估计对该产品的需求至少会增加100%。 设施吞吐量在12个月内完成。为了满足这一额外的需求 应用生物系统373 Plus DNA测序系统的能力是 已请求。该仪器由一个用于产生DNA的机器人组成 模板和荧光DNA测序仪分析反应 产品。该仪器将使DNA的容量增加一倍以上 作为机器人的排序设施可以用来为这两个人提供产品 新的和现有的荧光分析仪。对…的广泛兴趣 定义和操纵单个DNA片段意味着增加 DNA序列的生成速度和能力将使所有人受益 Img调查人员及其工作人员。

DNA sequence analysis is currently provided at the Institute for Molecular Genetics (IMG) at Baylor College of Medicine by a core facility. The sequencing core provides a mechanism whereby investigators can obtain DNA sequence information without having to dedicate their own laboratory resources. Fragments are provided to the facility in a variety of forms and in different DNA doning vectors, and the data is returned via a computer network. The sequencing capacity of the facility is almost saturated with the current 373A Applied Biosystems automated DNA sequencer operated on every working day and approximately 50% of weekend days. The methods for DNA template preparation and for manipulating the sequencing reaction components are now being simplified, and the demands from individual projects are increasing. We estimate a minimum of a 100% increase in demand for the facilities throughput within 12 months. To cater for this additional capacity an Applied Biosystems 373 Plus DNA Sequencing system is requested. The instrument consists of a robot for generating DNA templates and a fluorescent DNA sequencer to analyze the reaction products. The instrument will more than double the capacity of the DNA sequencing facility as the robot can be used to supply products for both the new and existing fluorescence analyzers. The wide interest in defining and manipulating individual DNA fragments means the increase in rate and capacity for the generation of DNA sequence will benefit all IMG investigators and their staff.