INTERACTIVE THREE-DIMENSIONAL GRAPHICS COMPUTER SYSTEM

我们建议增加一个计算机化的三维图像设施 重建和分析的细胞计数设施， UCI发育生物学中心（DBC）。 流式细胞仪 已经提供了激光扫描共聚焦显微镜技术， 流式细胞术、图像分析和操作以及单细胞 微注射的DBC成员以及一些外部用户。 的3D 成像设施将主要从堆叠的2D图像产生的工作， 荧光标记的整个组织样本的共聚焦显微镜， 使3D重建、旋转、伪彩色和体积成为可能 在几分钟内完成测量，而不是花费许多小时 使用现有技术。 该系统还将用于操纵 通过磁共振成像显微镜获得的图像集。 该设备最初将用于研究 果蝇胚胎和成虫盘中的细胞粘附分子， 海胆胚胎和胚胎中细胞谱系模式的分析 发展脊椎动物神经系统，绘图和实验 下丘脑神经元生长锥发育的研究 脊椎动物视觉系统，研究细胞发育方面 在躯体感觉皮层，负责进食的神经细胞 水螅的反应，以及识别神经元的电特性 在果蝇中。

We propose to add a facility for computerized three-dimensional image reconstruction and analysis to the Cytometric Facility at the Developmental Biology Center (DBC), UCI. The Cytometric Facility already provides the techniques of laser scanning confocal microscopy, flow cytometry, image analysis and manipulation, and single-cell microinjection for DBC members as well as some outside users. The 3D imaging facility will work primarily from stacked 2D images generated by confocal microscopy of fluorescent-labeled whole tissue samples and will make possible 3D reconstruction, rotation, pseudocoloring and volume measurements in a matter of minutes rather than the many hours consumed using present technology. The system will also be used to manipulate image sets obtained by magnetic resonance imaging microscopy. The equipment will be used initially in studies of the expression of cell adhesion molecules in Drosophila embryos and imaginal discs, analysis of cell lineage patterns in sea urchin embryos and in the developing vertebrate nervous system, mapping and experimental investigation of developing neuronal growth cones in the lower vertebrate visual system, investigating cellular aspects of development in the somatosensory cortex, the nerve cells responsible for the feeding response of hydra, and the electrical properties of identified neurons in Drosophila.