LASER SCANNING CONFOCAL MICROSCOPE

激光扫描共焦显微镜

• 批准号: 3521027
• 负责人: Anthony P. Bretscher
• 金额: $ 18.4万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-25 至 1992-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521027
• 关键词: biomedical equipment purchase; confocal scanning microscopy; cytology; fluorescence microscopy; immunofluorescence technique; lasers

This application is being submitted by a group of eight investigators who use immunofluorescence microscopy as a significant part of their research effort. All are using this technique to localize molecules that are spatially distributed through individual cells or in small animals. Wide field immunofluorescence microscopy has the disadvantage that light from above and below the plane of focus blurs the image and so limits the information that can be obtained by this technique. This technical problem has been overcome by the development of laser scanning confocal microscopy. We are requesting funds to purchase a Bio-Rad MRC-600 Confocal Fluorescence Imaging System and a dedicated Zeiss microscope. The eight laboratories (funded by 12 NIH grants) cover a large range of research topics, all of which would greatly benefit by the application of confocal microscopy. These topics include studies on the structure and assembly of the cytoskeleton (Drs. Bretscher and Quaroni), on the spatial and temporal organization of internal membranes (Dr. Brown), on the detailed structure of the mitotic spindle in wild-type and mutant Drosophila (Dr. Goldberg), on the cytoskeletal organization during early development in wild-type and mutant embryos of Caenorhabditis (Dr. Kemphues), on the distribution of proteins that regulate the cell cycle, cell-to-cell communication and cellular transformation (Dr. Shalloway), on the structure of the neuromuscular junction (Dr. Salpeter), and pronuclear fusion and secretory products of the male accessory gland in Drosophila (Dr. Wolfner). The proposed confocal microscope will be housed in the Flow Cytometry/Video Microscopy Facility of the Cornell University Biotechnology Program which will make available a full-time technician to run the instrument. Further technical support, should it be necessary, will be provided by the NIH BIomedical Research Technology Resource and NSF National Instrumentation Facility for Optical Microscopy at Cornell University, directed by Dr. Watt W. Webb.

这份申请是由八名调查人员提交的，他们 使用免疫荧光显微镜作为他们研究的重要部分 努力。所有人都在使用这项技术来定位分子 在空间上分布在单个细胞或小动物体内。宽 现场免疫荧光显微镜的缺点是来自 焦平面的上方和下方会模糊图像，因此限制了 可以通过这种技术获得的信息。这个技术问题 已经被激光扫描共聚焦显微镜的发展所克服。 我们正在申请购买Bio-Rad MRC-600共聚焦荧光仪的资金 成像系统和专用蔡司显微镜。八个实验室 (由美国国立卫生研究院12项拨款资助)涵盖了广泛的研究主题，所有 这将极大地受益于共焦显微镜的应用。 这些主题包括对结构和组装的研究 细胞骨架(Bretscher和Quaroni博士)，关于时空 内膜的组织(布朗博士)，关于详细结构 野生型和突变型果蝇的有丝分裂纺锤体(戈德堡博士)， 野生型和野生型早期发育过程中细胞骨架组织的研究 凯菲斯博士)的突变胚胎对线虫的分布 调节细胞周期、细胞间通讯和 细胞转化(Shalloway博士)，关于 神经肌肉连接(萨尔彼特博士)，以及原核融合和分泌 果蝇雄性附腺的产物(沃尔夫纳博士)这个 拟议的共焦显微镜将安装在流式细胞术/视频中 康奈尔大学生物技术计划的显微镜设施 将提供一名全职技术人员来操作该仪器。进一步 必要时，美国国立卫生研究院将提供技术支持 生物医学研究技术资源与NSF国家仪器 康奈尔大学光学显微镜设施，由瓦特博士指导 W·韦伯。