LASER SCANNING CONFOCAL MICROSCOPE

激光扫描共焦显微镜

• 批准号: 3521027
• 负责人: Anthony P. Bretscher
• 金额: $ 18.4万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-25 至 1992-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521027
• 关键词: biomedical equipment purchase; confocal scanning microscopy; cytology; fluorescence microscopy; immunofluorescence technique; lasers

This application is being submitted by a group of eight investigators who use immunofluorescence microscopy as a significant part of their research effort. All are using this technique to localize molecules that are spatially distributed through individual cells or in small animals. Wide field immunofluorescence microscopy has the disadvantage that light from above and below the plane of focus blurs the image and so limits the information that can be obtained by this technique. This technical problem has been overcome by the development of laser scanning confocal microscopy. We are requesting funds to purchase a Bio-Rad MRC-600 Confocal Fluorescence Imaging System and a dedicated Zeiss microscope. The eight laboratories (funded by 12 NIH grants) cover a large range of research topics, all of which would greatly benefit by the application of confocal microscopy. These topics include studies on the structure and assembly of the cytoskeleton (Drs. Bretscher and Quaroni), on the spatial and temporal organization of internal membranes (Dr. Brown), on the detailed structure of the mitotic spindle in wild-type and mutant Drosophila (Dr. Goldberg), on the cytoskeletal organization during early development in wild-type and mutant embryos of Caenorhabditis (Dr. Kemphues), on the distribution of proteins that regulate the cell cycle, cell-to-cell communication and cellular transformation (Dr. Shalloway), on the structure of the neuromuscular junction (Dr. Salpeter), and pronuclear fusion and secretory products of the male accessory gland in Drosophila (Dr. Wolfner). The proposed confocal microscope will be housed in the Flow Cytometry/Video Microscopy Facility of the Cornell University Biotechnology Program which will make available a full-time technician to run the instrument. Further technical support, should it be necessary, will be provided by the NIH BIomedical Research Technology Resource and NSF National Instrumentation Facility for Optical Microscopy at Cornell University, directed by Dr. Watt W. Webb.

该申请由八名调查员组成的小组提交，他们 使用免疫荧光显微镜作为他们研究的重要组成部分 努力。 所有人都在使用这种技术来定位分子 通过单个细胞或小动物的空间分布。 宽的 场免疫荧光显微镜的缺点是来自 焦点平面的上方和下方会使图像变得模糊，从而限制了 通过该技术可以获得的信息。 这个技术问题 激光扫描共焦显微镜的发展已经克服了这一问题。 我们正在申请资金购买 Bio-Rad MRC-600 共焦荧光仪 成像系统和专用蔡司显微镜。 八个实验室 （由 12 项 NIH 拨款资助）涵盖广泛的研究主题，所有 这将大大受益于共焦显微镜的应用。 这些主题包括对结构和组装的研究 细胞骨架（Bretscher 和 Quaroni 博士），关于空间和时间 内膜组织（布朗博士），关于详细结构 野生型和突变果蝇的有丝分裂纺锤体（Goldberg 博士）， 对野生型和早期发育过程中细胞骨架组织的影响 秀丽隐杆线虫（Caenorhabditis）突变体胚胎（Dr. Kemphues）的分布 调节细胞周期、细胞间通讯和 细胞转化（Shalloway 博士），关于细胞的结构 神经肌肉接头（Salpeter 博士）以及原核融合和分泌 果蝇雄性附属腺的产物（沃尔夫纳博士）。这 拟议的共焦显微镜将安装在流式细胞术/视频中 康奈尔大学生物技术项目的显微镜设施 将安排一名全职技术人员来运行该仪器。 更远 如有必要，将由 NIH 提供技术支持 生物医学研究技术资源和 NSF 国家仪器 康奈尔大学光学显微镜设施，由瓦特博士指导 W.韦伯.