MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE

酿酒酵母中的微丝

• 批准号: 6179548
• 负责人: Anthony P. Bretscher
• 金额: $ 26.21万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6179548
• 关键词: Golgi apparatus; Saccharomyces cerevisiae; actins; cellular polarity; fungal genetics; gene expression; gene mutation; immunoelectron microscopy; immunofluorescence technique; microfilaments; molecular cloning; molecular genetics; protein structure function; protein transport; secretory protein; tropomyosin

The goal of the proposed studies is to understand the molecular details responsible for the transport and targeting of secretory products via the actin cytoskeleton. Current results suggest that secretory vesicles are transported down actin cables by a myosin V. The proposed experiments explore how transport is affected in mutants with altered polarity and what features of the actin cytoskeleton are required to allow it to function as a myosin V substrate. Four specific aims are presented: (1) to develop a system for the localization of the sites of emergence of secretory products; (2) to produce temperature sensitive tropomyosin (tpm1) mutants that will be used to investigate polarization and depolarization of the actin cytoskeleton and secretory processes; (3) to screen for mutant that have a tpm1 sensitive secretory defect; and (4) to identify proteins that interact with the myosin V tail by biochemical and genetic approaches.

这项研究的目的是了解分子细节 负责分泌产物的运输和靶向， 肌动蛋白细胞骨架 目前的结果表明，分泌囊泡 被肌球蛋白V沿着肌动蛋白电缆运送。 实验探索了运输是如何影响突变体与改变 极性和肌动蛋白细胞骨架的什么特征是必需的， 使其作为肌球蛋白V底物发挥作用。 四个具体目标是 提出：（1）开发网站本地化系统 分泌产物的出现;（2）产生温度敏感的 原肌球蛋白（tpm 1）突变体，将用于研究极化 肌动蛋白细胞骨架和分泌过程的去极化; (3)筛选具有tpm 1敏感性分泌缺陷的突变体; 以及（4）通过以下方法鉴定与肌球蛋白V尾相互作用的蛋白质： 生物化学和遗传学方法。