ELECTRON MICROSCOPE FACILITY

此申请要求一半资金购买JEM-1200EXII 电子显微镜。 将提供显微镜的剩余费用 获得大学基础生命科学学院的配套资金 密苏里州堪萨斯城。 这将是多用户的一部分， 学校的多学科设施。 拥有联邦拨款的核心用户 支持将立即受益于该显微镜的存在，因为 该设备对于许多正在进行和计划中的项目非常重要 调查。 该仪器的可用性将有助于 研究：1.) 不同脊椎动物的肌肉粗丝结构 课程； 2.) 神经支配的结构框架 垂体中叶由含有 GABA 和/或 DA 的神经元组成； 3.)单个小脑神经元对乙醇的敏感性； 4.) 的 乙酰胆碱酯酶和胆碱能表达的机制 成人和发育过程中心脏的神经支配； 5.) 血红素和铁结合蛋白和运输； 6.) 病毒的组装 和核蛋白； 7.) 基底外侧膜中的离子通道 肾小管。 这些项目都将从中受益匪浅 基础生命科学学院有电子显微镜， 由于学校目前没有此类仪器，因此 不仅需要支持这些研究，而且还需要支持未来的研究 学院研究人员的快速增长将产生这种影响。 的 大学致力于支持以下领域的研究计划 学校，并将提供空间和必要的设施 设备的安装和长期运行。

This application requests one-half of the funds to purchase a JEM-1200EXII electron microscope. The remaining cost of the microscope will be provided in matching funds from the School of Basic Life Sciences at the University of Missouri at Kansas City. This will be part of a multiuser, multidisciplinary facility in the School. Core users with Federal grant support will immediately benefit from the presence of this microscope since this equipment is important to a number of on-going and planned investigations. The availability of this instrumentation will facilitate studies of: 1.) muscle thick filament structure in different vertebrate classes; 2.) the structural framework for the innervation of the intermediate lobe of the pituitary by GABA and/or DA containing neurons; 3.) the sensitivity to ethanol of single cerebellar neurons; 4.) the mechanisms involved in expression of acetylcholinesterase and cholinergic innervation of the heart, both in the adult and during development; 5.) heme and iron binding proteins and transport; 6.) the assembly of viruses and nucleoproteins; and 7.) ion channels in the basolateral membrane of renal tubules. These projects will all benefit significantly from the presence of an electron microscope in the School of Basic Life Sciences, since no such instrumentation is currently available in the School, and is vitally needed to support not only these studies but future studies which will arise from the rapid growth in research faculty in the School. The University is committed to the support of the research program in the School, and will provide both space and the necessary facilities for the installation and long-term operation of the equipment.