ELECTRON MICROSCOPE FACILITY

此申请要求一半的资金购买JEM-1200 EXII 电镜 显微镜的剩余费用将由 与大学基础生命科学学院的资金相匹配 在堪萨斯城的密苏里州。 这将是多用户的一部分， 学校的多学科设施。 获得联邦赠款的核心用户 支持将立即受益于这种显微镜的存在，因为 该设备对许多正在进行和计划进行的 调查事务所 这种仪器的可用性将有助于 研究：1.）不同脊椎动物肌肉粗丝结构 类; 2.神经支配的结构框架 含GABA和/或DA神经元的垂体中叶; 3.）第三章单个小脑神经元对乙醇的敏感性; 4.）的 参与乙酰胆碱酯酶和胆碱能神经元表达的机制 心脏的神经支配，无论是在成人和在发展过程中; 5.） 血红素和铁结合蛋白和运输; 6.）病毒的装配 和核蛋白;和7.）基底外侧膜离子通道 肾小管 这些项目都将大大受益于 基础生命科学学院的电子显微镜， 由于目前学校没有这样的仪器， 不仅需要支持这些研究，而且需要支持未来的研究， 将来自于学院研究人员的快速增长。 的 大学致力于支持研究计划， 学校，并将提供空间和必要的设施， 设备的安装和长期运行。

This application requests one-half of the funds to purchase a JEM-1200EXII electron microscope. The remaining cost of the microscope will be provided in matching funds from the School of Basic Life Sciences at the University of Missouri at Kansas City. This will be part of a multiuser, multidisciplinary facility in the School. Core users with Federal grant support will immediately benefit from the presence of this microscope since this equipment is important to a number of on-going and planned investigations. The availability of this instrumentation will facilitate studies of: 1.) muscle thick filament structure in different vertebrate classes; 2.) the structural framework for the innervation of the intermediate lobe of the pituitary by GABA and/or DA containing neurons; 3.) the sensitivity to ethanol of single cerebellar neurons; 4.) the mechanisms involved in expression of acetylcholinesterase and cholinergic innervation of the heart, both in the adult and during development; 5.) heme and iron binding proteins and transport; 6.) the assembly of viruses and nucleoproteins; and 7.) ion channels in the basolateral membrane of renal tubules. These projects will all benefit significantly from the presence of an electron microscope in the School of Basic Life Sciences, since no such instrumentation is currently available in the School, and is vitally needed to support not only these studies but future studies which will arise from the rapid growth in research faculty in the School. The University is committed to the support of the research program in the School, and will provide both space and the necessary facilities for the installation and long-term operation of the equipment.