400 MHZ WIDE BORE NMR SPECTROMETER

请求提供资金，以购置一台400兆赫宽口径核磁共振分光计， 支持高分辨率结构研究、细胞内代谢 使用灌注器官和细胞培养系统的研究， 大分子动力学的表征，以及体内成像， 在啮齿动物肿瘤模型上的光谱学。 所要求的仪器是 通用电气400 MHz多核NMR光谱仪， 可互换匀场和梯度设置， 液体工作和显微成像。 仪器将配置为 探针允许质子，氟，磷，碳，氮， 氯、氘、钠和其他原子核，温度可变 能力，一个阵列处理器，以允许快速处理图像， 2D数据集，以及宽带多普勒，以允许间接检测 X原子核。 此外，我们还将为特殊的 应用于四极核和大体积应用， 必要 本工具所服务的调查人员包括 生物物理学、药理学和生物化学教授。 此外 减轻IBM-270 MHz仪器的当前过载， 医学中心的化合物的结构表征，新的 仪器将支持关于新陈代谢代谢特征的工作 氟化合物，抗肿瘤药物，和表征 代谢和解剖反应的几种治疗策略。 在 此外，该仪器将支持阴离子的机理研究 膜系统中的传输，显微成像技术的发展， 和大分子动力学的表征。 共同文书 将被安置在电流共享的体内NMR光谱仪附近 生物物理系的设施。

Funds are requested for a 400 MHz wide-bore NMR spectrometer that will support high resolution structural studies, intracellular metabolic studies using perfused organs and cell culture systems, the characterization of macromolecular dynamics, and in vivo imaging and spectroscopy on rodent tumor models. The instrument requested is a General Electric 400 MHz multinuclear NMR spectrometer operating with an interchangeable shim and gradient set that permits both high resolution liquids work and micro-imaging. The instrument will be configured with probes to permit proton, fluorine, phosphorous, carbon, nitrogen, chlorine, deuterium, sodium, and other nuclei, variable temperature capabilities, an array processor to permit rapid handling of image and 2D data sets, and a broad band decoupler to permit indirect detection of X nuclei. In addition, we will construct probes for special applications to quadrupole nuclei and large volume applications as necessary. The investigators to be served by this instrument include professors in biophysics, pharmacology, and biochemistry. In addition to relieving the current excessive load on the IBM-270 MHz instrument in the Medical Center for structural characterization of compounds, the new instrument will support work on metabolic characterization of metabolism of fluorine compounds, of anti-tumor drugs, and characterization of metabolic and anatomic response to several therapeutic strategies. In addition the instrument will support mechanistic studies of anion transport in membrane systems, development of micro-imaging techniques, and characterization of macromolecular dynamics. The shared instrument will be housed adjacent to a current shared in vivo NMR spectrometer facility in the Biophysics Department.