Enhanced high-field nuclear magnetic resonance spectroscopy

增强高场核磁共振波谱

• 批准号: LE220100009
• 负责人: Prof Gottfried Otting
• 金额: --
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE220100009
• 关键词: Enhanced; field; nuclear; magnetic; resonance

Nuclear magnetic resonance (NMR) spectroscopy is the most powerful analytical tool in chemistry, ranging from small molecules to 3D structure determinations of large biomolecules such as proteins and DNA. This application is for a probe that offers the highest possible sensitivity of detection, about 2.5 times higher than the current probe, which is a loaned item and partly defect. The new probe is essential to regain full operation of the 800 MHz NMR spectrometer at the Australian National University, which constitutes a $4M investment. By accelerating measurements 6-fold and being much less sensitive to salt, a greater range of protein systems can be studied, including important drug targets such as the CoV-2 main protease.

核磁共振波谱是化学中最强大的分析工具，范围从小分子到蛋白质和DNA等生物大分子的三维结构测定。这项申请适用于提供最高检测灵敏度的探头，大约是当前探头的2.5倍，目前的探头是借来的物品，部分有缺陷。新的探测器对于恢复澳大利亚国立大学800 MHz核磁共振波谱仪的全面运行至关重要，这一投资高达400万美元。通过将测量速度加快6倍，并且对盐的敏感度大大降低，可以研究更大范围的蛋白质系统，包括重要的药物靶标，如CoV-2主要蛋白酶。