GUANINE NUCLEOTIDE BINDING PROTEINS AS RECEPTOR-EFFECTOR COUPLERS

鸟嘌呤核苷酸结合蛋白家族(G蛋白)在 作为受体-效应偶联剂的跨膜信号传递。G蛋白偶联 一系列不同的受体，包括激素的受体， 神经递质、光线、气味和某些生长因子。效应器 职能(积极的，在某些情况下是消极的)由 G蛋白包括cAMP形成、肌醇磷脂分解、钾 和钙通道，以及cGMP降解。我们使用了各种不同的 研究其表达、分布、调控、结构和功能的技术 G蛋白的功能。我们的研究突出了 G蛋白家族。使用多肽特异性抗体，我们已经定义了 G蛋白与受体和效应器偶联的特异性。我们有 明确明确的翻译后脂质修饰是必要的 G蛋白α和β/γ亚基的膜附着。我们有 在阿尔法亚基中产生突变，导致结构性激活，以及 将它们导入细胞以确定表型对细胞的影响 功能。这些研究为理解 G蛋白在正常信号转导中的作用及其可能的解释 G蛋白结构或功能缺陷是异常信号的基础 转导。

A family of guanine nucleotide binding proteins (G-proteins) functions in transmembrane signalling as receptor-effector couplers. G-proteins couple to a diverse array of receptors including those for hormones, neurotransmitters, light, odorants, and certain growth factors. Effector functions regulated (positively and, in some instances, negatively) by G-proteins include CAMP formation, phosphoinositide breakdown, potassium and calcium channels, and cGMP degradation. We have used a variety of techniques to study the expression, distribution, regulation, structure and function of G-proteins. Our studies highlight the diversity within the G-protein family. Using peptide specific antibodies, we have defined the specificity of G-proteins in coupling to receptors and effectors. We have defined distinct post-translational lipid modifications necessary for membrane attachment of G protein alpha and beta/gamma subunits. We have created mutations in alpha subunits that cause constitutive activation, and transfected these into cells to define phenotypic effects on cellular function. These studies provide the basis for understanding the role of G-proteins in normal signal transduction and for elucidating possible defects in G-protein structure or function as the basis for abnormal signal transduction.