GTP BINDING PROTEINS AND ADENYLATE CYCLASE
GTP 结合蛋白和腺苷酸环化酶
基本信息
- 批准号:3878895
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Cholera toxin, the secretory product of Vibrio cholerae responsible in part
for the devastating diarrheal syndrome characteristic of cholera, activates
adenylyl cyclase by catalyzing the ADP-ribosylation of Gs(alpha), the
stimulatory guanine nucleotide-binding protein of the cyclase system. This
toxin-catalyzed reaction is stimulated, in the presence of GTP, by about 20
kDa guanine nucleotidebinding proteins, termed ADP-ribosylation factors or
ARFs. Two forms of ARF, sARF I and sARF II, were isolated from bovine brain
cytosol. Rabbit polyclonal antibodies against bovine sARF II reacted with
soluble and membrane ARFs but did not react with other guanine
nucleotide-binding proteins such as the 20 kDa protein ras and the
heterotrimeric transducing G proteins (e.g., Gt(alpha), Gs(alpha),
Gi(alpha), and Go(alpha)). The anti-ARF antibodies recognized about 20 kDa
ARF-like proteins in a variety of species and organ systems. The highest
levels of immunoreactivity were observed in brain and other neural tissues.
In these tissues an ARF doublet was observed, with the upper band (sARF II)
being the predominant form. In other tissues, an immunoreactive band
corresponding to the lower molecular weight species (sARF I) was present at
higher concentration.
Levels of ARF, when quantified by immunoreactivity, correlated with those
determined by a functional assay, stimulation of cholera toxin-catalyzed
ADP-ribosylation. During rat brain development, when quantified by both
immunoreactivity and function, sARF II was lowest at birth, showed some
increase at 10 days, and was maximal at 27-60 days; sARF I was unchanged.
In spleen and heart, sARF I predominated over sARF II; in spleen, it
increased with age while in heart, it decreased. Based on these studies, it
appears that ARF proteins share epitopes and are expressed at different
levels during development.
霍乱毒素是霍乱弧菌的分泌产物,
霍乱的致命性霍乱综合征,
腺苷酸环化酶通过催化Gs(α)的ADP-核糖基化,
环化酶系统的刺激性鸟嘌呤核苷酸结合蛋白。这
在GTP存在下,约20
kDa鸟嘌呤核苷酸结合蛋白,称为ADP-核糖基化因子或
急性呼吸衰竭从牛脑中分离到两种形式的ARF,sARF I和sARF II,
胞质液兔抗牛sARF II多克隆抗体与
可溶性和膜性ARF,但不与其他鸟嘌呤反应
核苷酸结合蛋白如20 kDa蛋白ras和
异源三聚体转导G蛋白(例如,Gt(α),Gs(α),
Gi(alpha)和Go(alpha))。抗ARF抗体识别的分子量约为20 kDa
ARF样蛋白在多种物种和器官系统中。最高
在脑和其他神经组织中观察到免疫反应水平。
在这些组织中,观察到ARF双峰,上部条带(sARF II)
成为主导形式。在其他组织中,免疫反应带
对应于较低分子量物质(sARF I),
更高的浓度。
当通过免疫反应性定量时,ARF水平与那些
通过功能测定确定,刺激霍乱毒素催化的
ADP-核糖基化。在大鼠大脑发育过程中,
免疫反应性和功能,sARF II在出生时最低,显示出一些
10天时增加,27-60天时达到最大值; sARF I无变化。
在脾脏和心脏中,sARF I高于sARF II;在脾脏中,
随着年龄的增长而增加,而在心脏中,则下降。根据这些研究,
似乎ARF蛋白共享表位,并在不同的
发展过程中的水平。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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