MOLECULAR BIOLOGY OF THE GENES ENCODING PROHORMONES FOR BOMBESIN-LIKE PEPTIDES

这个项目的目标是了解结构的各个方面， 哺乳动物同源基因的功能和调控 到蛙毒素样神经肽。两种哺乳动物蛙素样多肽 到目前为止，已被发现的胃泌素释放肽(MP和Neuromedin B (NM-B)。这两种多肽都存在于中枢和外周的一部分。 神经元，并在它们的羧基末端结构域共享一个结构基序， 这是与高亲和力细胞结合的必要条件和充分条件 表面蛙皮素受体。有几个子项目正在积极进行中 追求：1)大鼠和人前GRP基因和基因组克隆 前-NMB基因已被分离和鉴定 启动子区域的潜在调控基序。2)就地 杂交技术正被用来确定定位 GRP和NMB mRNA在细胞水平的表达；3)启动子-报告 通过以下方式将基因构建引入培养的细胞宿主 基因转染法定位人GRP基因启动子区域的元件 负责细胞类型特异性调控的基因。

The goal of this project is to understand aspects of the structure, function, and regulation of the genes encoding the mammalian counterparts to bombesin-like neuropeptides. Two mammalian bombesin-like peptides have been identified to date, gastrin-releasing peptide (MP and neuromedin B (NM-B). Both peptides are found in a subset of central and peripheral neurons, and share a structural motif at their carboxyl-terminal domains, which is necessary and sufficient for binding to high-affinity cell surface bombesin receptors. There are several subprojects being actively pursued: 1) CDNA and genomic clones for rat and human prepro-GRP and prepro-NMB genes have been isolated and characterized to identify potential regulatory motifs in the promoter regions. 2) in situ hybridization techniques are being utilized to determine the localization of GRP and NMB mRNA at the cellular level in brain; 3) Promoter-reporter gene constructs will be introduced into cultured cell hosts by transfection to map the elements in the promoter region of the human GRP gene responsible for the cell-type specific regulation.