BIOMEDICAL RESEARCH SUPPORT GRANT

生物医学研究资助

• 批准号: 3517113
• 负责人: JAMES L MATTHEWS
• 金额: $ 1.74万
• 依托单位: BAYLOR RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3517113
• 关键词: health science research support

A transmission electron microscope is requested to be used by four PHS-supported projects. One of these projects includes an ultrastructural examination of the neurons involved in bladder function while the other three address issues of mammalian CNS regeneration. Project 1 (CM) examines the structural basis for urination and employs iontophoretic filling of sacral preganglionic neurons with horseradish peroxidase. One-third of this project will be devoted to an electron microscopic analysis of these neurons and the types of synaptic inputs they receive. The results of this project could benefit victims of spinal cord injury by providing insights into ways to restore lost bladder control. Project 2 (FL) looks at the role of astrocytes in inhibiting adult mammalian CNS regeneration. The model used is the adult mammalian dorsal root transitional zone where regenerating axons grow from a supportive PNS environment into an inhibitory CNS one. The major thrust of this project includes a detailed ultrastructural analysis of the interactions of growing axons with reactive astrocytes in this region of the spinal cord. Understanding how astrocytes stop axonal growth could lead to interventions that may promote axonal regeneration after human CNS injury. Project 3 (KS) asks whether CNS axons can innervate peripheral targets, thereby restoring motor and sensory function . While it has been shown that CNS axons will enter and grow in peripheral nerve grafts, it has not been clearly demonstrated whether these axons can establish a functional innervation of skin and muscle. This project combines neurophysiological and light and electron microscopic approaches. Reinnervation of peripheral targets could lead to ways of restoring sensation and some voluntary motor control to the body below the site of a spinal cord injury. 4 (KS) confronts Project questions of whether astrocytes may play a role in the remission of symptoms associated with multiple sclerosis. This project uses physiological techniques to evaluate axonal conduction through experimentally produced demyelinating lesions. Use of electron microscopy to establish a set of ultrastructural correlates that describe recovery of function is an essential adjunct to the physiological experiments as a way of characterizing the cellular constitution of the lesion sites, and the presence and type of any intracellular contacts. Data from this project will contribute to a better understanding of the cellular processes occurring within central demyelinating lesions and how these processes affect the course of disease such as multiple sclerosis.

传输电子显微镜要求四个 PHS支持的项目。这些项目之一包括超微结构 检查与膀胱功能相关的神经元，而另一个 三个解决了哺乳动物CNS再生的问题。项目1（CM）检查 排尿的结构基础，并采用离子遗迹填充 s骨前神经元与辣根过氧化物酶。其中三分之一 项目将专门用于对这些项目的电子显微镜分析 神经元及其收到的突触输入的类型。结果的结果 项目可以通过提供见解来使脊髓损伤的受害者受益 进入恢复失去膀胱控制的方法。项目2（FL）着眼于角色 星形胶质细胞抑制成年哺乳动物中枢神经系统再生。模型 使用的是成年哺乳动物背根过渡区 再生轴突从支撑性PNS环境中生长到 抑制性中枢神经系统。该项目的主要目的包括一个详细的 对生长轴突与反应性的相互作用的超微结构分析 在脊髓的该区域中的星形胶质细胞。了解星形胶质细胞 停止轴突生长可能导致可能促进轴突的干预措施 人类中枢神经系统受伤后的再生。项目3（KS）询问CNS轴突是否 可以支配外围目标，从而恢复电动机和感觉 功能 。虽然已经证明CNS轴突将进入并成长 周围神经移植物，尚未清楚地证明 轴突可以建立皮肤和肌肉的功能神经支配。这 项目结合了神经生理学和光和电子微观 方法。重新支配外围目标可能导致 恢复感觉和一些自愿运动控制到下方的身体 脊髓损伤的部位。 4（ks）面对项目问题是否存在 星形胶质细胞可能在减轻与 多发性硬化症。该项目使用生理技术来评估 通过实验产生脱髓鞘病变的轴突传导。 使用电子显微镜建立一组超微结构相关性 描述功能的恢复是对 生理实验是表征细胞的一种方式 病变部位的构成以及任何的存在和类型 细胞内触点。该项目的数据将有助于更好 了解中央内发生的细胞过程 脱髓鞘病变以及这些过程如何影响疾病进程 例如多发性硬化症。