BIOMEDICAL RESEARCH SUPPORT

生物医学研究支持

• 批准号: 3516849
• 负责人: HARRY B. KOSTENBAUDER
• 金额: $ 2.32万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3516849
• 关键词: health science research support; university

In this institutional application by Boston University School of Medicine, we propose to establish a unified instrument facility bringing together existing equipment (oligonucleotide synthesizer, amino acid analyzers) and adding new state-of-the-art equipment in a core with trained personnel who will provide a service to a variety of research projects. We are requesting funding to purchase a peptide synthesis system with three reaction vessels and an HPLC system for peptide purification. The instruments will be utilized by four major research groups: the Cardiovascular Institute, and the Departments of Medicine, Biochemistry, and Biophysics. Major uses will involve: 1) synthesis of angiotensin inhibitor, synthesis of agonist and antagonist of bradykinin and vasopressin receptors (Dr. Gavras); 2) synthesis of peptides recognized by the platelet (GPIIb-IIIa) and other cell adhesion receptors termed integrins, synthesis of peptides corresponding to the platelet receptor binding domains of fibronectin, plasmin, urokinase, tissue plasminogen activator and thrombospondin (Dr. Plow); 3) synthesis of peptides corresponding to structural or functional domains of apolipoproteins (Dr. Small and associates). The instruments will be installed in the newly created molecular biology research facilities constructed with NIH funding (HL35669). The P.I. will be responsible for the overall operation of these instruments. Dr. Lammek, an accomplished peptide chemist will be responsible for the daily operation of the peptide synthesizer and the HPLC. After two years of support from the University, most of the financial support for the operation of the instruments will be obtained from existing or new research grants. Research activities have expanded dramatically at the School of Medicine in the past few years with several new faculty recruits. Emphasis has been placed on the utilization of molecular biology approaches to study problems pertinent to hypertension, atherosclerosis, and pulmonary disease.

在波士顿大学医学院的这份机构申请中， 我们建议建立一个统一的仪器设施， 现有设备（寡核苷酸合成仪、氨基酸分析仪）和 在核心中增加新的最先进的设备，并配备训练有素的人员， 将为各种研究项目提供服务。我们要求 资金用于购买具有三个反应容器的肽合成系统 和用于肽纯化的HPLC系统。 这些工具将 由四个主要研究小组使用：心血管研究所， 医学系、生物化学系和生物物理系。主要用途将 涉及：1）血管紧张素抑制剂合成，激动剂的合成， 缓激肽和加压素受体拮抗剂（Gavras博士）; 2） 血小板识别肽（GPIIb-IIIa）的合成及其他 称为整合素的细胞粘附受体，肽的合成 对应于纤连蛋白的血小板受体结合结构域， 纤溶酶，尿激酶，组织纤溶酶原激活剂和血小板反应蛋白（博士。 Plow）; 3）合成对应于结构或功能的肽 载脂蛋白结构域（Small博士和同事）。器械将 安装在新创建的分子生物学研究设施中， 由NIH资助（HL 35669）构建。私家侦探将负责 这些工具的整体运作。拉马克博士，一位多才多艺的 肽化学家将负责肽的日常操作 合成器和HPLC。经过两年的大学支持， 对这些文书运作的大部分财政支助将由 从现有或新的研究赠款中获得。研究活动 在过去的几年里， 几个新来的教职员工重点放在利用 分子生物学的方法来研究相关的问题， 高血压、动脉粥样硬化和肺病。