AMINO ACID ANALYZER AND UPDATE OF PROTEIN SEQUENCER

我们建议更换一部无法维修的氨基酸分析仪，并提升 蛋白质测序仪，以保持最先进的仪器， 我们的蛋白质化学实验室6个主要用户和至少4个次要用户 将在各种项目中使用所要求的工具。1)的 核仁RNA相关蛋白的结构与功能关系 将被研究。2)辅因子连接的序列、位点和区域 电子载体蛋白质的蛋白质间相互作用 测定3)髓磷脂蛋白P（O）的功能特性将是 研究了4)翻译后修饰语的结构和作用 真核生物蛋白质合成起始和延伸因子 将被阐明。5)鱼类免疫球蛋白的许多结构将被 测定6)新分离细菌的氨基酸含量 将分析铁载体。其他应用程序将包括质量 化学合成肽的控制，精确的蛋白质浓度 生物物理学研究的测定，摇蚊的特征 唾液腺分泌多肽，mRNA结合的部分测序 蛋白质和氨酰tRNA上tRNA结合位点的鉴定 合成酶

We propose to replace an unrepairable amino acid analyzer and to upgrade a protein sequencer in order to maintain state-of-the-art instrumentation in our Protein Chemistry Facility. Six primary and at least 4 secondary users will utilize the requested instruments in a variety of projects. 1) The relationship of structure to function of nucleolar RNA associated proteins will be studied. 2) The sequences, sites of cofactor attachment and regions of protein-protein interactions of electron carrier proteins will be determined. 3) The functional properties of myelin protein P(O) will be investigated. 4) The structures and roles of posttranslational modifica- tions of eukaryotic protein synthesis initiation and elongation factors will be elucidated. 5) The many structures of fish immunoglobulins will be determined. 6) The amino acid content of newly isolated bacterial siderophores will be analyzed. Additional applications will include quality control of chemically synthesized peptides, accurate protein concentration determinations for biophysical studies, characterization of Chironomus salivary gland secretion polypeptides, partial sequencing of mRNA binding proteins and identification of tRNA binding sites on aminoacyl tRNA synthetases.