ONTOGENY AND REGIONAL DISTRIBUTION OF NEUROPEPTIDE MRNAS IN RODENT BRAIN

前缩胆囊素和前脑啡肽的个体发育研究项目已经完成 完成。同样的cdna探针也被用来研究区域 前缩胆囊素和脑啡肽原在啮齿动物体内的分布 大脑。 前胆囊收缩素原mRNA和胆囊收缩素多肽水平分别为 用RNA印迹分析在大鼠和小鼠脑的多个区域进行测量 和放射免疫分析。与脑啡肽原基因表达水平的比较 同样的区域也被制作出来。

The project on ontogeny of preprocholecystokinin and proenkephalin has been completed. The same cDNA probes have been used to study the regional distribution of preprocholecystokinin and proenkephalin mRNAs in rodent brain. The levels of preprocholecystokinin mRNA and cholecystokinin peptide were measured in several regions of rat and mouse brain using RNA blot analysis and radioimmunoassay. Comparison with proenkephalin mRNA levels in the same regions was made.