PURIFICATION OF MESSENGER RNAS ENCODING FOR NEUROTROPIC FACTORS IN RAT BRAIN

神经营养因子已被证明出现在大鼠大脑中 损伤后。 该项目旨在分子克隆 基因编码这些病变诱导的营养因子， 个脑袋 首先，我们开发了产生这些因子的方法， 检测它们的神经营养活性 一小部分mRNA， 从受损的大鼠大脑中制备，负责神经营养 通过在非洲爪蟾体内翻译成蛋白质， Laevis卵母细胞。 这个分数现在被用来构建一个 cDNA文库在载体pGEM blue中的表达。 我们还建立了一个 大鼠脑损伤特异性cDNA文库的构建 13. history of life 通过差异菌落杂交，我们得到 选择的阳性克隆，其中一些已被测试， 编码神经营养活性

Neurotrophic factors have been shown to appear in the rat brain after lesion. This project intends the molecular cloning of the gene encoding for these lesion-induced trophic factors in the brain. First, we developed the assays to produce these factors and test for their neurotrophic activity. A fraction of mRNA, prepared from lesioned rat brain, responsible for neurotrophic activity was found by in vivo translation into proteins in Xenopus Laevis oocytes. This fraction is now being used to construct a cDNA library in the vector pGEM blue. We also constructed a cDNA library specific for lesioned rat brain in the vector bluescribe M 13. By differential colony hybridization, we have selected positive clones and some of these have been tested for coding for neurotrophic activity.