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PURCHASE OF HIGH FIELD NMR SPECTROMETER

购买高场核磁共振波谱仪

基本信息

批准号：
3520277
负责人：
GERD N LA MAR
金额：
$ 40万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-05-08 至 1991-12-01
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3520277
关键词：
biochemistry biomedical equipment purchase chemical structure function conformation imaging /visualization /scanning membrane proteins molecular biology nuclear magnetic resonance spectroscopy

项目摘要

This grant proposal requests partial funding for a vertical bore, high field NMR spectrometer for high resolution and in-vivo spectroscopy of biomolicules, model compounds, and peerfused tissues and small mammals. The instrument will consist of the highest proven field magnet, homogeneous magnetic field diameter of at least 10mm, and a console with all features necessary for advanced techniques such as multidimensional and multiple quantum experiments, proton detection of heteronuclei, spin locking, and capagility of generating pulse sequences of complex pulse phase. The spectrometer will be utilized by a core group of eight investigators (Primary Users) organized into three research areas, as will as several secondary users. The individual projects of the investigators cover a wide range of fundamental research in molecular biology and biochemistry. Every investigator has an established program of NMR research with spectrometers of lesser capability and the proposal documents the additional knowledge to be gained by the new capabilities of the proposed instrument. Areas of research to be studied utilizing the proposed spectrometer include structure and dynamics of biomolecules, in-vivo biochemistry, model compound structure, and the relationship between molecular conformation and biological function. Specific topics include (in part) heme protein structure and dynamics, histone structure, renal physiology, drugs effective in AIDS treatment, and conformation of cell surface polysaccharides, as well as several other fundamental studies in molecular biology and biochemistry. The proposed instrument will come under the auspices of the UCD NMR Facility. The Facility has an established record over ten yeears in enabling investigators to implement state of the art NMR techniques, and in spectrometer maintenance and modification.

这项拨款申请要求为垂直钻孔提供部分资金，用于高分辨率和体内高场NMR光谱仪生物分子、模型化合物和渗透的光谱学组织和小型哺乳动物。该文书将包括：经验证的最高磁场磁体，均匀磁场直径至少10毫米，以及一个控制台，先进的技术，如多维和多量子实验，异质核的质子探测，自旋锁定，具有产生复相位脉冲序列的能力。光谱仪将由八人核心小组使用研究人员（主要用户）分为三个研究领域，以及几个次要用户。的个别项目研究人员涵盖了广泛的基础研究，分子生物学和生物化学。每个调查员都有建立了核磁共振研究的程序，能力和建议书记录了其他知识，通过拟议仪器的新功能获得。利用拟议的光谱仪进行研究的领域包括生物分子结构和动力学生物化学，模型化合物结构，分子构象和生物功能之间的联系具体主题包括（部分）血红素蛋白质结构和动力学，组蛋白结构，肾脏生理学，艾滋病药物处理和细胞表面多糖的构象，如以及其他几项分子生物学基础研究，生物化学拟议的仪器将在UCD NMR的主持下进行设施。该设施有一个既定的记录超过十年使研究人员能够实施最先进的核磁共振技术技术，以及光谱仪的维护和修改。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Elucidation of fipronil photodegradation pathways.

DOI：
10.1021/jf9913007
发表时间：
2000-09
期刊：
Journal of agricultural and food chemistry
影响因子：
6.1
作者：
K. Ngim;S. Mabury;D. Crosby
通讯作者：
K. Ngim;S. Mabury;D. Crosby

1H NMR study of the dynamics of the pH modulation of axial coordination in Aplysia limacina (Val(E7)) and sperm whale double mutant His(E7)-->Val,Thr(E10)-->Arg metmyoglobin.

海兔 (Val(E7)) 和抹香鲸双突变体 His(E7)-->Val,Thr(E10)-->Arg 高铁肌红蛋白轴向配位 pH 调节动力学的 1H NMR 研究。

DOI：
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Qin,J;Pande,U;LaMar,GN;Ascoli,F;Ascenzi,P;Cutruzzolá,F;Travaglini-Allocatelli,C;Brunori,M
通讯作者：
Brunori,M

Identification of residues in the aromatic substrate binding site of horseradish peroxidase by 1H NMR studies on isozymes.

通过同工酶的 1 H NMR 研究鉴定辣根过氧化物酶芳香底物结合位点中的残基。