CONFOCAL SCANNING MICROSCOPE FACILITY

生物系内的共焦扫描显微镜设备 将作为共享设施，主要面向 发育生物学亚组。虽然光学显微镜的最新进展 开辟了许多新的研究途径，新的仪器是 费用昂贵；该基金的目标将是实现有效利用， 为参与者提供最大限度地使用这些工具的机会 科学家。这里需要资金购买一台新的扫描共聚焦 用于荧光显微镜和用于光学存储盘记录器。这个 设施还将容纳部门的蔡司Axiomat，它配备了 用于显微注射。该设施将由私家侦探萨拉博士监督 C.R.Elgin，在熟练的技术人员Michael Veith的帮助下，以及 将被安置在与实验室空间一致的新空间中 发育生物学亚组。计算机接口和软件 发展部将由詹姆斯·麦克纳利博士执导，他拥有一名初选 在生物医学计算领域的任命。主要研究项目有哪些 将利用的设施有以下几点：1)角色分析 染色质结构在基因表达控制中的作用(S.C.R.Elgin)。 显微镜将被用来分析某些物质的分布 多线染色体中的染色体蛋白质和测定 某些染色体蛋白质在多线体中结合的时间 并确定某些染色体联合的时间 果蝇早期胚胎中有核的染色体蛋白。2) Volvox(D.L.Kirk)细胞分裂器的分析。这些 研究的目的是了解不对称分裂的机制 将大的不朽生殖细胞与小的人类区分开来 体细胞。3)果蝇的形态调控分析(I.M. 邓肯)。计划进行详细的研究，以确定 福氏塔拉苏和双胸肌蛋白产物在胚胎中的定位 通过免疫荧光技术。4)花纹形成机理分析 苍白多孔菌(P.G.McNally)构造格局的分布 蛋白质，包括细胞骨架的元素，将被分析以 确定细胞移动是对 图案机。所有这些研究都将提供基本的 关于生长和发展机制的信息。

The Confocal Scanning Microscope Facility within the Department of Biology will serve as shared facility, oriented primarily to the needs of the developmental biology subgroup. While recent advances in light microscopy have opened many new avenues of research, the new instruments are expensive; the goal of the Facility will be to achieve efficient use, providing maximum access to these instruments for the participating scientists. Funds are requested here for a new scanning confocal fluorescence microscope and for an optical memory disc recorder. The Facility will also house the Departmental Zeiss Axiomat, which is equipped for microinjection. The Facility will be supervised by the P.I., Dr. Sarah C.R. Elgin, with the assistance of a skilled technician, Michael Veith, and will be housed in new space congruent with the laboratory space of the developmental biology subgroup. Computer interfacing and software developmental will be directed by Dr. James McNally, who holds a primary appointment in Biomedical Computing. The major research projects which will make use of the facility are the following: 1) Analysis of the role of chromatin structure in the control of gene expression (S.C.R. Elgin). The microscope will be used to analyze the distribution of certain chromosomal proteins in the polytene chromosomes and to determine the timing of association of certain chromosomal proteins in the polytene chromosomes and to determine the timing of association of certain chromosomal proteins with nuclei in the early embryo of Drosophila. 2) Analysis of the cell division apparatus in Volvox (D.L. Kirk). These studies are aimed at understanding the mechanism of asymmetric division that differentiates the large immortal germ cells from the small mortal somatic cells. 3) Analysis of morphological regulation in Drosophila (I.M. Duncan). Detailed studies are planned to establish the precise localization of fushi tarazu and bithorax protein products in embryo whole mounts by immunofluorescence. 4) Analysis of the patterning mechanism of Polysphondylium pallidum (J.G. McNally). The distribution of structural proteins, including elements of the cytoskeleton, will be analyzed to determine whether cell movement is a response to or an actual component of the patterning machine. All of these studies will provide basic information of mechanisms of growth and development.