CLINICAL LUNG CANCER RESEARCH PROJECT

临床肺癌研究项目

• 批准号: 3939576
• 负责人: T C MCLEMORE
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939576
• 关键词: cell bank /registry; drug design /synthesis /production; drug screening /evaluation; eicosanoid metabolism; human tissue; lung neoplasms; neoplasm /cancer chemotherapy; neoplasm /cancer classification /staging; respiratory pharmacology; tissue /cell culture

The development of therapeutic modalities for the treatment of human lung cancer has, to date, been disappointing. Classification of lung tumors with regard to their metabolic potential is particularly important for developing a systematic approach for the identification of active anti-lung tumor agents. We have, therefore, initiated this project in order to develop a relevant and feasible plan for classification of human lung tumors, as well as for identification of effective new lung cancer treatment modalities. This project will interface the clinical aspects of human lung cancer with basic science and applied science technologies which are currently available in an effort to provide a comprehensive program for characterization of lung tumors based on their morphological, ultrastructural, biochemical, metabolic, and molecular genetic characteristics as well as establish in vitro and in vivo techniques for investigating potential antitumor drugs against primary human lung tumors. The initial results from our laboratories document the feasibility and relevance of this project by demonstrating: (1) considerable variation in prostanoid and 4-ipomeanol metabolism as well as cytokeratin expression among individual fresh human lung tumor specimens as well as lung tumor cell lines, (2) the ability to establish and propagate primary lung tumor cell cultures which will provide material for in vitro drug screening, establishing long term tumor cell lines, and for comparative biochemical studies, (3) the ability to propagate human lung tumors in the lungs of nude mice by novel intrabronchial and intrathoracic implantation methods which allow for improved in vivo propagation, biochemical and drug screening studies. An ultimate goal of this project is to provide a paradigm for individualized lung cancer characterization and pharmacoselective drug treatment based on the biochemical profiles of individual lung tumors and corresponding normal lung tissue.

发展治疗方式， 迄今为止，人类肺癌的研究成果令人失望。 肺肿瘤的代谢分类 潜力对于发展一个系统的 用于鉴定活性抗肺肿瘤剂的方法。 因此，我们启动了这一项目，以开发一个 为人类肺部肿瘤的分类提供了相关可行的方案， 以及用于鉴定有效的新肺癌 治疗方式。 该项目将与临床 人类肺癌的基础科学和应用 目前可用的科学技术， 提供了一个全面的程序，用于表征肺 基于肿瘤的形态学、超微结构、生物化学 代谢和分子遗传特征以及 建立体外和体内技术， 抗肿瘤药物对原发性人肺肿瘤的作用。 初始 我们实验室的结果证明了可行性， 该项目的相关性，通过展示：（1）相当大的 前列腺素和4-异戊烯醇代谢的变化以及 人新鲜肺肿瘤细胞角蛋白表达 标本以及肺肿瘤细胞系，（2） 建立和繁殖原代肺肿瘤细胞培养物， 将为体外药物筛选提供材料， 术语肿瘤细胞系，以及用于比较生物化学研究， (3)人类肺肿瘤在肺中传播的能力 裸小鼠支气管内和胸腔内植入 允许改进体内繁殖的方法， 生化和药物筛选研究。 这个项目的最终目标 该项目旨在为个体化肺癌提供一个范例， 表征和药物选择性药物治疗， 单个肺肿瘤的生化特征， 对应的正常肺组织。