CLINICAL LUNG CANCER RESEARCH PROJECT

临床肺癌研究项目

• 批准号: 3963319
• 负责人: T C MCLEMORE
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963319
• 关键词: drug screening /evaluation; eicosanoid metabolism; human tissue; lung neoplasms; neoplasm /cancer chemotherapy; neoplasm /cancer classification /staging; tissue /cell culture

The development of therapeutic modalities for the treatment of human lung cancer has, to date, been disappointing. Classification of lung tumors with regard to their metabolic potential is particularly important for developing a systematic approach for the identification of active antilung tumor agents. We have, therefore, initiated this project in order to develop a relevant and feasible plan for classification of human lung tumors, as well as for identification of effective new lung cancer treatment modalities. This project will interface the clinical aspects of human lung cancer with basic science and applied science technologies which are currently available in an effort to provide a comprehensive program for characterization of lung tumors based on their morphological, ultrastructural, biochemical, metabolic, and molecular genetic characteristics as well as establish in vitro and in vivo techniques for investigating potential antitumor drugs against primary human lung tumors. The initial results from our laboratories document the feasibility and relevance of this project by demonstrating: (1) considerable variation in prostanoid as well as 4-ipomeanol metabolism among individual fresh human lung tumor specimens, (2) the ability to establish and propagate primary lung tumor cell cultures which will provide material for in vitro drug screening, establishing long term tumor cell lines, and for comparative biochemical studies, (3) the ability to propagate human lung tumors in the lungs of nude mice by a novel intrabronchial implantation method which will allow for improved in vivo propagation and drug screening studies. An ultimate goal of this project is to provide a paradigm for individualized lung cancer characterization and treatment as an improvement over current approaches.

人类肺部疾病治疗模式的发展 迄今为止，癌症一直令人失望。 肺肿瘤的分类 关于它们的代谢潜力， 开发一种系统的方法来识别活性抗肺 肿瘤因子 因此，我们启动了这一项目，以便 制定相关且可行的人体肺部分类计划 肿瘤，以及用于识别有效的新肺癌 治疗方式。 该项目将接口的临床方面， 人类肺癌基础科学和应用科学技术， 目前正在努力提供一个全面的计划， 基于它们的形态学表征肺肿瘤， 超微结构、生化、代谢和分子遗传学 特性以及建立体外和体内技术， 研究针对原发性人肺肿瘤的潜在抗肿瘤药物。 我们实验室的初步结果证明了可行性， 该项目的相关性，通过证明：（1）在 新鲜人前列腺素和4-异戊烯醇代谢 肺肿瘤标本，（2）建立和传播原发性 肺肿瘤细胞培养物将为体外药物 筛选，建立长期肿瘤细胞系，并用于比较 生物化学研究，（3）人类肺肿瘤在肿瘤细胞中增殖的能力， 通过一种新的支气管内植入方法， 允许改进的体内繁殖和药物筛选研究。 一个 该项目的最终目标是为个性化提供一个范例， 肺癌的表征和治疗是对当前 接近。