USE OF RETROVIRAL SHUTTLE VECTOR FOR INFECTION OF ONCOGENES INTO HUMAN CELLS

携带有正义或反义方向的病毒 已经产生了以下基因：v-raf、c-raf、v-Ha-ras、c-ras、c-raf、v-Ha-ras、c-ras、c-ras。 MYC(小鼠)和PDGF A链(正常和羧基缺失 顺反子)。Ras、v-raf和PDGF A链的正义结构 在小鼠3T3细胞上均能产生转化灶。多数 在裸鼠体内产生了潜伏期相对较短的肿瘤。 Zip-Ha-ras病毒感染永生化人支气管 上皮细胞(BEAS 12)。皮下注射后5周内 500万细胞感染裸鼠，裸鼠体内出现肿瘤 80%的老鼠。这些培养细胞的核型显示 他们在起源上是人类。 用Zip-Ha-sar病毒(ras反义)感染TBE-1细胞 (转化的原代人支气管上皮细胞 在它们是v-Ha-ras癌基因必需的之后)。我们得出结论 从这个实验来看，Ha-ras基因的功能是必要的 为了维持TBE-1细胞自感染以来的增殖， 应该在G418的存在下成长，不要因此而 功能的反义废除。Zip-Ha-sar感染细胞 缺乏之前引入的v-Ha-ras mRNA，而是 代表sar转录本的预期5.4kb信使核糖核酸。

Viruses carrying either sense or anti-sense orientations of the following genes have been produced: v-raf, c-raf, v-Ha-ras, c- myc(mouse), and PDGF A chain (normal and carboxyl-deleted cistrons). The sense constructs of ras, v-raf, and PDGF A chain were found to produce transformed foci on mouse 3T3 cells. Most produced tumors in nude mice with relatively short latencies. Zip-Ha-ras virus was used to infect immortalized human bronchial epithelial cells (Beas 12). Within 5 weeks from subcutaneous infection of 5 million cells into nude mice, tumors appeared in 80% of the mice. The karyotype of these cultured cells shows them to be human in origin. Zip-Ha-sar virus (ras anti-sense) were used to infect TBE-1 cells (primary human bronchial epithelial cells which were transformed after they were essential with a v-Ha-ras oncogene). We conclude from this experiment that the Ha-ras gene function is necessary to maintain TBE-1 cell proliferation since infected cells, which should grow in the presence of G418, do not as a consequence of anti-sense abrogation of function. The Zip-Ha-sar infected cells lack the previously introduced v-Ha-ras mRNA and have instead the expected 5.4-kb mRNA representing the sar transcript.