CARDIAC MYOCYTES EXPRESS ACIDIC AND BASIC FGF

酸性和碱性成纤维细胞生长因子(aFGFs和bFGFs)是 有丝分裂多肽，也有血管生成，神经营养，和 形态发生活动。在大多数组织中都发现了它们，但 它们在体内的细胞来源尚不清楚。大约一半的人 所有间充质细胞在培养中都会合成碱性成纤维细胞生长因子，少数 这些细胞在培养中合成aFGF。然而，体外培养 表达可能不是体内情况的真实指标。 缺乏免疫细胞化学或原位杂交 这些多肽和基因分别阻止了它们的 细胞定位。 我们采取了另一种方法，用酶法分离心肌细胞。 消化。然后对这些细胞进行清洗、裂解并进行 肝素琼脂糖层析法。洗脱出来的分数 然后检测aFGFs和bFGFs的位置特性 3T3细胞的有丝分裂活性及免疫反应性检测 斑点印迹法检测酸性和碱性成纤维细胞生长因子。两种化验结果都表明 这些多肽在心肌细胞中含量丰富。角色 在幼年成年大鼠心肌细胞中，这些多肽是 不分裂且不受病理性肥大或 血管生成，将需要进一步的研究。

Acidic and basic fibroblast growth factors (aFGF and bFGF) are mitogenic peptides which also have angiogenic, neurotropic, and morphogenic activities. They have been found in most tissues but their cellular origin in vivo is unknown. Approximately half of all mesenchymal cells synthesize bFGf in culture and a minority of these cells synthesize aFGF in culture. However, the in vitro expression may not be a true indicator of the situation in vivo. The absence of immunocytochemistry or in situ hybridization for these peptides and genes, respectively, has prevented their cellular localization. We took another approach and isolated cardiac myocytes by enzymatic digestion. These cells were then washed, lysed and subjected to heparin sepharose chromatography. The fractions which elute in the positions characteristic of aFGF and bFGF were then tested for mitogenic activity on 3T3 cells and tested for immunoreactivity for acidic and basic FGF by dot blot. Both types of assays suggest that these peptides are abundant in cardiac myocytes. The roles of these peptides in young adult rat cardiac myocytes, which are not dividing and not subject to pathologic hypertrophy or to angiogenesis, will require further investigation.