CARDIAC MYOCYTES EXPRESS ACIDIC AND BASIC FGF

心肌细胞表达酸性和碱性 FGF

• 批准号: 3920219
• 负责人: M S LEE
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3920219
• 关键词: fibroblast growth factor; heart cell; laboratory rat; mitogens; tissue /cell culture

Acidic and basic fibroblast growth factors (aFGF and bFGF) are mitogenic peptides which also have angiogenic, neurotropic, and morphogenic activities. They have been found in most tissues but their cellular origin in vivo is unknown. Approximately half of all mesenchymal cells synthesize bFGf in culture and a minority of these cells synthesize aFGF in culture. However, the in vitro expression may not be a true indicator of the situation in vivo. The absence of immunocytochemistry or in situ hybridization for these peptides and genes, respectively, has prevented their cellular localization. We took another approach and isolated cardiac myocytes by enzymatic digestion. These cells were then washed, lysed and subjected to heparin sepharose chromatography. The fractions which elute in the positions characteristic of aFGF and bFGF were then tested for mitogenic activity on 3T3 cells and tested for immunoreactivity for acidic and basic FGF by dot blot. Both types of assays suggest that these peptides are abundant in cardiac myocytes. The roles of these peptides in young adult rat cardiac myocytes, which are not dividing and not subject to pathologic hypertrophy or to angiogenesis, will require further investigation.

酸性和碱性成纤维细胞生长因子（aFGF和bFGF）是 促有丝分裂肽也具有促血管生成、促神经生长和 形态发生活动 它们存在于大多数组织中， 它们在体内的细胞来源是未知的。 的大约一半 所有的间充质细胞在培养中合成bFGF， 这些细胞在培养物中合成aFGF。 但是经体外 表达可能不是体内情况的真实指示。 缺乏免疫细胞化学或原位杂交， 这些肽和基因分别阻止了它们的 细胞定位 我们采用了另一种方法，通过酶促分离心肌细胞， 消化. 然后将这些细胞洗涤、裂解并经受 肝素琼脂糖色谱法。 其中， 然后检测aFGF和bFGF的特征性位置， 对3T3细胞的促有丝分裂活性，并检测免疫反应性 斑点杂交法检测酸性和碱性成纤维细胞生长因子。 这两种类型的分析表明， 这些肽在心肌细胞中大量存在。 的角色 这些肽在年轻的成年大鼠心肌细胞， 不分裂，不受病理性肥大或 血管生成，将需要进一步的研究。