High Throughput Screening of Pharmaceuticals Targeting Heart Cell Contractile Function

针对心脏细胞收缩功能的药物的高通量筛选

基本信息

  • 批准号:
    9347047
  • 负责人:
  • 金额:
    $ 20.51万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-04-01 至 2018-06-30
  • 项目状态:
    已结题

项目摘要

The isolated cardiac myocyte represents the smallest fully functional model system of heart muscle. Cardiac myocytes contract in response to electrical stimulation and are useful to characterize heart function in terms of contractility, calcium ion regulation, and action potential. Currently, the number of cardiac myocytes that can be examined and analyzed using current techniques is on the order of 1 myocyte per 10 min. Higher throughput is required, however, to accommodate faster drug discovery and a growing motivation in basic research toward large scale rapid data collection and analysis. This grant is focused on validating the feasibility of fully-automated identification of non-overlapping viable myocytes and quantification of contractility and calcium ion regulation in at least 100 myocytes in 10 min. In the pharmaceutical setting, this is a significant enhancement in phenotype quantification that expedites assessment of drug efficacy/toxicity and will greatly reduce costs by permitting companies to eliminate more dangerous or marginal compounds. In Aim 1, we will develop a lightweight x-y moveable inverted microscope that will scan a field of isolated cardiac myocytes. The goal of Aim 1 is to identify isolated and viable cardiac myocytes by their morphology and sarcomeric pattern such that at least 100 useable myocytes are identified within 10 min. In Aim 2, a novel image analysis method will quantify position, size, orientation and dynamic characteristics of contraction-relaxation function for all isolated cardiac myocytes within the field of view, again with the goal of 100 myocytes within 10 min. IonOptix is located in Massachusetts and has provided microscopy-based equipment for assessing intracellular calcium regulation and contraction/relaxation function for the last 25 years.
离体心肌细胞是最小的全功能心肌模型系统。

项目成果

期刊论文数量(0)
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BRADLEY M PALMER其他文献

BRADLEY M PALMER的其他文献

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{{ truncateString('BRADLEY M PALMER', 18)}}的其他基金

Ventricular and Cardiac Fiber Characterization and Integration Core
心室和心脏纤维表征和集成核心
  • 批准号:
    8215312
  • 财政年份:
    2011
  • 资助金额:
    $ 20.51万
  • 项目类别:
XANES OF ZINC BINDING TO CARDIAC PROTEINS
锌的 XAN 与心脏蛋白质的结合
  • 批准号:
    8361294
  • 财政年份:
    2011
  • 资助金额:
    $ 20.51万
  • 项目类别:
Ventricular and Cardiac Fiber Characterization and Integration Core
心室和心脏纤维表征和集成核心
  • 批准号:
    7789878
  • 财政年份:
    2010
  • 资助金额:
    $ 20.51万
  • 项目类别:
Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
心肌细胞锌及其收缩舒张功能的调节
  • 批准号:
    8290231
  • 财政年份:
    2008
  • 资助金额:
    $ 20.51万
  • 项目类别:
Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
心肌细胞锌及其收缩舒张功能的调节
  • 批准号:
    7812055
  • 财政年份:
    2008
  • 资助金额:
    $ 20.51万
  • 项目类别:
Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
心肌细胞锌及其收缩舒张功能的调节
  • 批准号:
    7661470
  • 财政年份:
    2008
  • 资助金额:
    $ 20.51万
  • 项目类别:
Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
心肌细胞锌及其收缩舒张功能的调节
  • 批准号:
    7523376
  • 财政年份:
    2008
  • 资助金额:
    $ 20.51万
  • 项目类别:
Ventricular and Cardiac Fiber Characterization and Integration Core
心室和心脏纤维表征和集成核心
  • 批准号:
    8432074
  • 财政年份:
  • 资助金额:
    $ 20.51万
  • 项目类别:
Ventricular and Cardiac Fiber Characterization and Integration Core
心室和心脏纤维表征和集成核心
  • 批准号:
    8611950
  • 财政年份:
  • 资助金额:
    $ 20.51万
  • 项目类别:
Ventricular and Cardiac Fiber Characterization and Integration Core
心室和心脏纤维表征和集成核心
  • 批准号:
    8374705
  • 财政年份:
  • 资助金额:
    $ 20.51万
  • 项目类别:

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