ANALYTICAL TRANSMISSION ELECTRON MICROSCOPE

分析透射电子显微镜

• 批准号: 3519896
• 负责人: MILES A CRENSHAW
• 金额: $ 24.7万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-11 至 1989-04-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519896
• 关键词: biomedical equipment purchase; dental research; electron microscopy; image processing; online computer; scanning electron microscopy

This proposal seeks partial support for a Philips CM 12/STEM electron microscope to replace an AEI 801 instrument in the Dental Research Center (DRC) at the University of North Carolina. The DRC was established in 1967 and is one of five NIH funded regional dental research institutes in the nation. Currently, the DRC's electron microscope laboratory houses both SEM and TEM instruments and related technical facilities. The School of Dentistry and its DRC are deeply involved in basic research, with annual grant/contract acquisitions in excess of 4.2 million dollars. In the basic sciences, the DRC operates nationally recognized research programs in mineralized tissue, embryology, teratology, cell biology, collagen biochemistry, hemostasis, neurophysiology, and biomaterials. The School offers graduate research training in oral biology and in eight clinical fields. The AEI 801 was purchased in 1971. Maintained on annual service contract since that time, the instrument can produce reasonable images to 40-60,000X magnification. However, by present standards our AEI is sharply limited as a research instrument which in turn has severely hampered the DRC's current research programs. First, by constantly pressing against the AEI's magnification limit, investigator manuscripts are being returned by editors demanding superior micrographs. Second, our AEI lacks the microprocessor controlled analytical capabilities that are now considered standard for advanced level work, including X-ray microanalysis, electron diffraction, and image processing. Third, our AEI is difficult and expensive to maintain and common problems (alignment drift, vacuum, electrical), together with instrument limitations, actively discourage its use. Fourth, availability and access to other EM facilities on the UNC campus are limited and expensive, primarily because of intensive use these instruments already receive. To move forward by overcoming the four problems identified, the Philips CM 12/STEM is proposed as an instrument with superior resolution, clean ultrahigh vacuum, full analytical capabilities, and microprocessor controlled imaging and operating mode features.

该提案寻求对Philips CM 12/STEM的部分支持 电子显微镜取代AEI 801仪器 北方大学牙科研究中心（DRC） 卡罗莱纳。 DRC成立于1967年，是五个NIH之一。 资助了全国的地区牙科研究机构。 目前，刚果民主共和国的电子显微镜实验室既有 SEM和TEM仪器及相关技术设施。 的 牙科学院及其DRC深入参与基础 研究，每年获得的赠款/合同超过4.2 百万美元。 在基础科学领域，DRC 国家认可的矿化组织研究项目， 胚胎学，畸形学，细胞生物学，胶原生物化学， 止血、神经生理学和生物材料。 学校提供 口腔生物学研究生培训和八个临床 领域的 AEI 801于1971年购买。 每年维持 服务合同自签订之日起， 40-60，000倍放大的合理图像。 但到 目前的标准，我们的AEI作为一项研究是非常有限的 这反过来又严重阻碍了刚果民主共和国的 目前的研究计划。 首先，通过不断施压， AEI的放大倍数限制，调查人员的手稿正在 编辑们要求提供上级显微照片，并返回。 二是我们 AEI缺乏微处理器控制的分析能力 现在被认为是高级工作的标准， 包括X射线微区分析、电子衍射和图像 处理. 第三，我们的AEI维护起来困难且昂贵 和常见问题（对准漂移、真空、电气）， 加上仪器的限制，积极劝阻其使用。 第四，是否有其他机电设施，以及能否使用这些设施 校园是有限的和昂贵的，主要是因为密集的 使用这些工具已经收到。 向前移动 飞利浦CM 12/STEM解决了上述四个问题， 建议作为一种具有上级分辨率、干净 全真空、全分析能力和微处理器 受控成像和操作模式特征。

项目成果

Runx2 controls a feed-forward loop between androgen and prolactin-induced protein (PIP) in stimulating T47D cell proliferation.

• DOI: 10.1002/jcp.22966
• 发表时间: 2012-05
• 期刊: JOURNAL OF CELLULAR PHYSIOLOGY
• 影响因子: 5.6
• 作者: Baniwal, Sanjeev K.;Little, Gillian H.;Chimge, Nyam-Osor;Frenkel, Baruch
• 通讯作者: Frenkel, Baruch

Genome-wide scan of resistin mRNA expression in omental adipose tissue of baboons.

狒狒网膜脂肪组织中抵抗素 mRNA 表达的全基因组扫描。

• DOI: 10.1038/sj.ijo.0802699
• 发表时间: 2005
• 期刊: International journal of obesity (2005)
• 作者: Tejero,ME;Cole,SA;Cai,G;Peebles,KW;Freeland-Graves,JH;Cox,LA;Mahaney,MC;Rogers,J;VandeBerg,JL;Blangero,J;Comuzzie,AG
• 通讯作者: Comuzzie,AG