INTERMEDIATE VOLTAGE ELECTRON MICROSCOPE

中压电子显微镜

• 批准号: 3519914
• 负责人: JON CLIFFORD LEWIS
• 金额: $ 30万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519914
• 关键词: biomedical equipment purchase; biomedical equipment resource; electron microscopy; immunocytochemistry

Proposed is the acquisition of an intermediate voltage electron microscope which will be equipped for microanalytical X-ray analysis, T.V. camera imaging and direct video output for computer image storage, processing, and analysis. The instrument will be incorporated into the electron microscopy laboratory maintained as an institutional resource by the Department of Pathology. As a resource, the instrument will be used by investigators from throughout Bowman Gray School of Medicine and Wake Forest University. This orientation is reflected in research projects involving investigators from 6 academic projects. In addition to the 6 core projects, 11 supplemental projects from 5 other departments at Bowman Gray School of Medicine and one project describing studies at the University of North Carolina are listed to document breadth of applicable research and potential for regional orientation. The six core projects describe experiments which capitalize on the unique characteristics of increased beam penetration and greater resolution with thick sections and whole-mount preparations. These characteristics will enhance studies of cellular events such as lysosome organization, surface receptor location, host cell- parasite interactions, cytoskeletal superstructure, and cell/matrix interactions in tissues. A major feature of the core projects is the unique correlative and often multiple use of 3-D microscopy, image processing/reconstruction, cytochemistry, immunocytochemistry, and microanalysis enabling better understanding of spatial questions relating cell and tissue organization to biochemistry, function and pathobiology.

建议获得一个中等电压的电子 将配备用于微分析X射线的显微镜 分析、电视摄像机成像和直接视频输出 计算机图像存储、处理和分析。这台仪器 将被并入电子显微镜实验室 作为一种机构资源，由新闻部维持 病理学。作为一种资源，该仪器将由 来自鲍曼·格雷医学院的研究人员 和维克森林大学。这一方向反映在 研究项目涉及来自6个学术机构的研究人员 项目。除了6个核心项目外，还有11个补充项目 鲍曼·格雷学院其他5个系的项目 医学和一个项目描述了在华盛顿大学的研究 北卡罗来纳州列出了适用的文件范围 区域定位的研究和潜力。六核 项目描述了利用独特的 射束穿透增加和更大的特性 厚切片和整装制剂的分辨率。 这些特征将加强对细胞事件的研究，如 作为溶酶体组织，表面受体位置，宿主细胞- 寄生虫相互作用、细胞骨架超结构和细胞/基质 组织中的相互作用。核心项目的一大特点是 3-D显微镜独特的相关性和经常的多用途， 图像处理/重建、细胞化学、 免疫细胞化学和微量分析使更好的 关于细胞和组织的空间问题的理解 组织到生物化学、功能和病理生物学。