SCANNING ELECTRON MICROSCOPE

建议获取一个高分辨率的扫描电子 显微镜将配备多功能探测器，用于 二次和背向散射电子成像，并将配置 为了允许信号混合、反转和细节增强，请将 视频信号与未处理信号的导数。这些 与低电压操作的容量相结合的功能将 满足整个机构的迫切需求，如六项所示 美国国立卫生研究院资助核心研究项目。核心项目包括 来自五个学术部门的研究人员，这些研究包括 四个不同的生物医学领域，包括：(1)视网膜再生 损伤后，(2)动脉粥样硬化的发病机制，(3)细胞和 血栓形成和溶栓中的生化事件，(4)感觉(听力) 因外淋巴瘘造成的损失。在细胞和亚细胞 这些项目解决了内皮细胞周转、神经元 细胞外基质在细胞迁移和分化中的作用 细胞调节和细胞表面受体的分布 血小板活化和巨噬细胞泡沫细胞形成。这些不同的 问题将利用扫描电子的不同特征 精密常心倾斜式显微镜用于 与纤维蛋白/血小板相互作用相关的空间(3维)问题 血栓形成和细胞外基质的组织 血管内皮细胞和平滑肌细胞表面的成分。这个 背向散射探测器将与放射自显影和 胶体金化学法测定脂蛋白受体分布 心血管疾病中的细胞损伤和周转；它也将被用于 在描述细胞外基质细胞表面受体的研究中 多酶促凝血剂复合体的成分和 血小板。其中四个项目将严重依赖于 为实现两个或多个共同定位而设计的实验中的信号集成 细胞和组织表面的受体或配体。仪器将会是 合并到机构电子显微镜实验室，该实验室 由病理学系负责维护。在一个 机构设施，该工具将向许多人提供 来自全校的调查人员。

Proposed is the acquisition of a high resolution scanning electron microscope which will be equipped with a multifunctional detector for secondary and backscatter electron imaging and which will be configured to allow signal mixing, inversion and detail enhancement by mixing the derivative of the video signal with the unprocessed signal. These features, when combined with the capacity for low voltage operation, will meet pressing needs throughout the institution as exemplified in the six NIH funded core research projects. The core projects involve investigators from five academic departments, and the studies encompass four distinct biomedical areas including: (1) retinal regeneration following injury, (2) pathogenesis of atherosclerosis, (3) cellular and biochemical events in thrombosis and thrombolysis, (4) sensory (hearing) loss due to perilymphatic fistulas. At the cellular and sub-cellular levels the projects address questions of endothelial turnover, neuronal migration and differentiation, the role of the extracellular matrix in cell regulation, and the distribution of surface receptors during platelet activation and macrophage foam cell formation. These diverse questions will draw upon different features of the scanning electron microscope with the precision eucentric tilt microstage being used for spatial (3-dimensional) questions related to fibrin/platelet interaction during thrombogenesis and the organization of extracellular matrix components at the surface of endothelial and smooth muscle cells. The backscatter detector will be used in conjunction with autoradiography and gold colloid chemistry to determine lipoprotein receptor distribution and cell damage and turnover in cardiovascular disease; it will also be used in studies describing cell surface receptors for extracellular matrix components and for components of the multienzyme procoagulant complex on platelets. Four of the projects will rely heavily upon the capacity for signal integration in experiments designed to co-localize two or more receptors or ligands on cell and tissue surfaces. The instrument will be incorporated into the institutional electron microscopy laboratory, which is maintained in the Department of Pathology. Within the context of an institutional facility, the instrument will be available to numerous investigators from throughout the University.