SCANNING ELECTRON MICROSCOPE

扫描电子显微镜

• 批准号: 3521301
• 负责人: JON CLIFFORD LEWIS
• 金额: $ 12.9万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-10 至 1992-05-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521301
• 关键词: atherosclerosis; autoradiography; binding proteins; biomedical equipment purchase; biomedical equipment resource; biomedical facility; cardiovascular disorder; cell migration; cellular pathology; extracellular matrix; fibrin; image enhancement; platelet activation; retina; scanning electron microscopy; thrombosis

Proposed is the acquisition of a high resolution scanning electron microscope which will be equipped with a multifunctional detector for secondary and backscatter electron imaging and which will be configured to allow signal mixing, inversion and detail enhancement by mixing the derivative of the video signal with the unprocessed signal. These features, when combined with the capacity for low voltage operation, will meet pressing needs throughout the institution as exemplified in the six NIH funded core research projects. The core projects involve investigators from five academic departments, and the studies encompass four distinct biomedical areas including: (1) retinal regeneration following injury, (2) pathogenesis of atherosclerosis, (3) cellular and biochemical events in thrombosis and thrombolysis, (4) sensory (hearing) loss due to perilymphatic fistulas. At the cellular and sub-cellular levels the projects address questions of endothelial turnover, neuronal migration and differentiation, the role of the extracellular matrix in cell regulation, and the distribution of surface receptors during platelet activation and macrophage foam cell formation. These diverse questions will draw upon different features of the scanning electron microscope with the precision eucentric tilt microstage being used for spatial (3-dimensional) questions related to fibrin/platelet interaction during thrombogenesis and the organization of extracellular matrix components at the surface of endothelial and smooth muscle cells. The backscatter detector will be used in conjunction with autoradiography and gold colloid chemistry to determine lipoprotein receptor distribution and cell damage and turnover in cardiovascular disease; it will also be used in studies describing cell surface receptors for extracellular matrix components and for components of the multienzyme procoagulant complex on platelets. Four of the projects will rely heavily upon the capacity for signal integration in experiments designed to co-localize two or more receptors or ligands on cell and tissue surfaces. The instrument will be incorporated into the institutional electron microscopy laboratory, which is maintained in the Department of Pathology. Within the context of an institutional facility, the instrument will be available to numerous investigators from throughout the University.

提出的是收购高分辨率扫描电子 显微镜，将配备多功能探测器， 二次和反向散射电子成像， 为了允许信号混合、反转和细节增强， 将视频信号与未处理的信号进行微分。 这些 当与低电压操作能力相结合时， 满足整个机构的迫切需要，如六个 NIH资助的核心研究项目。 核心项目包括 来自五个学术部门的研究人员，研究包括 四个不同的生物医学领域，包括：（1）视网膜再生 损伤后，（2）动脉粥样硬化的发病机制，（3）细胞和 血栓形成和血栓溶解中的生化事件，（4）感觉（听力） 外淋巴瘘导致的损失。 在细胞和亚细胞 水平的项目解决的问题，内皮细胞的周转，神经元 迁移和分化，细胞外基质的作用， 细胞调节和表面受体的分布 血小板活化和巨噬细胞泡沫细胞形成。 这些不同 问题将利用扫描电子的不同特征， 具有精密共心倾斜微工作台的显微镜用于 与纤维蛋白/血小板相互作用相关的空间（三维）问题 在血栓形成和细胞外基质的组织化过程中 在内皮细胞和平滑肌细胞的表面的组分。 的 反向散射探测器将与放射自显影术结合使用， 金胶体化学测定脂蛋白受体分布， 心血管疾病中的细胞损伤和周转;它也将用于 在描述细胞外基质的细胞表面受体的研究中， 组分和多酶促凝血复合物的组分 血小板 其中四个项目将严重依赖以下能力： 在设计用于共定位两个或更多个 细胞和组织表面的受体或配体。 仪器将 纳入机构电子显微镜实验室，其中 保存在病理科。 的上下文内 机构设施，该文书将提供给许多 整个大学的调查员。