MOLECULAR BIOLOGY OF NEUROTRANSMITTER RECEPTORS

神经递质受体的分子生物学

• 批准号: 3945307
• 负责人: J C VENTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3945307
• 关键词: aminoacid; beta adrenergic agent; brain cell; developmental genetics; evolution; gene expression; gene mutation; genetic transduction; human tissue; ligands; molecular biology; molecular cloning; molecular genetics; mutagens; mutant; neurophysiology; nucleic acid sequence; species difference; tissue /cell culture

Recently, we published a paper on Cloning and Sequence Analysis of the Human Brain beta-Adrenergic Receptor (FEBS Lett. 211:200-206, 1987). In a recent review concerning the evolution of adrenergic and cholinergic receptors we further postulated that the muscarinic cholinergic receptor has existed for over 600 million years (Prog. Neurobiol., in press) Pharmacological and biochemical evidence suggests that the beta-adrenergic receptor appeared later in evolution raising the possibility that it evolved as a result of gene duplication. In order to further answer questions concerning receptor evoltion we are in the process of cloning beta-adrenergic and muscarinic cholinergic receptors from shark and Drosophila tissue. The cloning of neural receptor genes also allowed us to study the structure-function relationship of these receptors by site-directed mutagenesis. We have successfully expressed the human beta2-adrenergic receptor gene is a mouse cell line previously lacking the functional receptor. A series of single amino acid substitution mutants have also been made and are in the process of being characterized. Study of these mutants should help us to locate those important residues responsible for ligand binding, signal transduction or maintenance of structural integrity. *(Transferred from LNP on 7/87).

最近，我们发表了一篇关于克隆和序列分析的论文