HUMAN BRAIN CDNA PROJECT

人脑 CDNA 项目

• 批准号: 3846276
• 负责人: J C VENTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3846276
• 关键词: biochemical evolution; brain; complementary DNA; computer assisted sequence analysis; developmental neurobiology; gene expression; genetic library; genome; human genetic material tag; molecular cloning; nucleic acid sequence; protein sequence; protein structure

The goal of this project is to isolate, sequence, and characterize the genes expressed in human brain. As many as half of the almost 50,000 human genes are believed to be expressed in the brain. While sequencing half of the human genetic material is expected to take over a decade, and coding regions in the resultant genomic sequence may not be clearly discerned, sequencing a large number of cDNA clones can readily provide coding sequence data. We are building a large library of sequences of human brain cDNA clones. The availability of a broad-based library of cDNA sequences will facilitate identification of coding regions in genomic sequences as well as providing a starting point for individual cloning projects. A variety of approaches are being used to select brain-specific clones and to eliminate highly represented sequences. Over eight thousand human brain genes have been identified by this method. Computer analysis of DNA and predicted protein sequences were performed to search for the presence of conserved primary structure motifs and relationships to previously sequenced genes. It was found that over half of the sequences represented new genes with no detectable similarity to previously sequenced genes. An additional percentage represent the human homolog of genes that have been sequenced in other organisms. Further characterization of several interesting clones with potential roles in neural development is currently in progress and will include chromosome localization, examination of tissue distribution of expression, functional analysis, and evolutionary conservation.

该项目的目的是隔离，序列和表征 在人脑中表达的基因。 近50,000个中的多达一半 人类基因被认为在大脑中表达。 测序时 预计一半的人类遗传物质将花费十年多，并且 所得基因组序列中的编码区可能不清楚 识别，测序大量的cDNA克隆可以轻松提供 编码序列数据。 我们正在建造一个大的序列库 人脑cDNA克隆。 一个基于广泛的库的可用性 cDNA序列将促进鉴定编码区域 基因组序列以及为个体提供起点 克隆项目。 正在使用各种方法来选择 大脑特异性克隆并消除高度代表的序列。 已经确定了超过八千个人脑基因 方法。 DNA和预测蛋白序列的计算机分析是 执行以寻找保守的主要结构的存在 基序和与先前测序基因的关系。 发现它 超过一半的序列代表了未检测到的新基因 与先前测序的基因相似。 额外的百分比 代表已经在其他中测序的基因的人类同源物 有机体。 进一步表征了几个有趣的克隆 目前正在进行神经发展中的潜在作用，将 包括染色体定位，检查组织分布 表达，功能分析和进化保护。