HUMAN BRAIN CDNA PROJECT

人脑 CDNA 项目

• 批准号: 3846276
• 负责人: J C VENTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3846276
• 关键词: biochemical evolution; brain; complementary DNA; computer assisted sequence analysis; developmental neurobiology; gene expression; genetic library; genome; human genetic material tag; molecular cloning; nucleic acid sequence; protein sequence; protein structure

The goal of this project is to isolate, sequence, and characterize the genes expressed in human brain. As many as half of the almost 50,000 human genes are believed to be expressed in the brain. While sequencing half of the human genetic material is expected to take over a decade, and coding regions in the resultant genomic sequence may not be clearly discerned, sequencing a large number of cDNA clones can readily provide coding sequence data. We are building a large library of sequences of human brain cDNA clones. The availability of a broad-based library of cDNA sequences will facilitate identification of coding regions in genomic sequences as well as providing a starting point for individual cloning projects. A variety of approaches are being used to select brain-specific clones and to eliminate highly represented sequences. Over eight thousand human brain genes have been identified by this method. Computer analysis of DNA and predicted protein sequences were performed to search for the presence of conserved primary structure motifs and relationships to previously sequenced genes. It was found that over half of the sequences represented new genes with no detectable similarity to previously sequenced genes. An additional percentage represent the human homolog of genes that have been sequenced in other organisms. Further characterization of several interesting clones with potential roles in neural development is currently in progress and will include chromosome localization, examination of tissue distribution of expression, functional analysis, and evolutionary conservation.

本项目的目标是分离、测序和表征 人类大脑中表达的基因。 将近5万人中的一半 人类基因被认为是在大脑中表达的。 测序时 一半的人类遗传物质预计需要十多年的时间， 所得基因组序列中的编码区可能不清楚 可以看出，测序大量的cDNA克隆可以很容易地提供 编码序列数据。 我们正在建立一个大型的序列库， 人脑cDNA克隆。 提供基础广泛的图书馆， cDNA序列将有助于识别编码区， 基因组序列以及提供一个起点， 克隆项目。 各种各样的方法被用来选择 大脑特异性克隆和消除高度代表性序列。 超过8000个人类大脑基因已经被鉴定出来 法 计算机分析DNA和预测的蛋白质序列， 进行搜索保守的一级结构的存在 基序和与先前测序基因的关系。 发现 超过一半的序列代表了新的基因， 与先前测序的基因相似。 另一百分比 代表在其他基因组中已被测序的人类基因同源物， 有机体 进一步表征几个感兴趣的克隆， 神经发育中的潜在作用目前正在进行中，并将 包括染色体定位、组织分布检查 表达、功能分析和进化保守。