CISPLATIN KINETICS

化学的实验和理论研究 顺铂(DDP)一直在继续，目标是 表征药物在体外的反应动力学。 已经确定了主要的反应物种和途径 在药物反应的前24小时内，各种缓冲 无机溶液和血浆超滤液。 药物水合/水解产物的分离 由反相高效液体实现 层析-电化学检测。家长， 单水型和双水型药物可以通过这种方式检测到 技术。量化评估已通过以下方式得到增强 计算色谱峰下的面积，而不是 根据峰值高度的不同， 该化合物的峰出现后的单水测量 在氧气峰的肩上。 实验结果表明，DDP与纯净的 水的降解速度接近其在血浆中的降解速度 超滤液，尽管后者的氯离子含量很高 解决方案。虽然一些血浆反应性可以归因于 小分子量亲核试剂，如蛋氨酸和 可能是重碳酸盐，

Experimental and theoretical studies on the chemistry of cisplatin (DDP) have been continued with the goal of characterizing the reaction kinetics of the drug in vitro. Principal reactive species and pathways have been identified over the first 24 hours of drug reaction in various buffered inorganic solutions and plasma ultrafiltrates. Separation of drug aquation/hydrolysis products has been accomplished by reverse phase high-performance liquid chromatography with electrochemical detection. Parent, monoaquo and diaquo drug forms are detectable by this technique. Quantitative assessment has been enhanced by computing areas under chromatographic peaks rather than depending on peak heights, a significant improvement for monoaquo measurement since the peak of this compound appears on the shoulder of an oxygen peak. Experimental results have shown that DDP reacts with pure water at a rate close to that of its degradation in plasma ultrafiltrate, despite the high chloride content of the latter solution. While some of the plasma reactivity is attributable to small molecular weight nucleophiles such as methionine and perhaps bicarbonate, the