UW X-RAY DIFFRACTOMETER AND AREA DETECTOR

请求提供资金，用于建立X射线快速数据收集设施， 衍射，其中将包括一个国家的最先进的区域检测器， 一种新的衍射仪与密封管源。 该设备将 是用于收集X射线的主要数据收集设施 氧化和还原形式的高分辨率衍射数据 蓝铜蛋白，铁硫蛋白，抗肿瘤蛋白 有和没有DNA嵌入剂结合， 氧结合蛋白血红素的氧化形式， 结合的变构效应物，硫肌红蛋白，铁氧还蛋白还原酶， 在非天然宿主中表达的人蛋白质，和蛋白质，如 三斜溶菌酶（X射线精加工技术试验用 基于衍射的模型）。 通过缩短收集数据所需的时间， 从而减少数据收集中的严重系统误差， 系统将加快结构研究在更高的分辨率， 稳定的化合物，使分析更大的 比我们以前研究的大分子。

Funds are requested for a rapid data collection facility for X-ray diffraction which will include a state-of-the-art area detector and a new diffractometer with a sealed tube source. The device will be the primary data collection facility used to collect X-ray diffraction data at high resolution on oxidized and reduced forms of blue copper proteins, iron-sulfur proteins, antitumor proteins with and without DNA intercalators bound to them, different oxygenated forms of the oxygen binding protein hemerythrin with allosteric effectors bound, sulfmyoglobin, ferredoxin reductase, human proteins expressed in unnatural hosts, and proteins such as triclinic lysozyme (for testing of refinement techniques of X-ray diffraction based models). By reducing the length of time necessary to collect data and thereby reducing serious systematic errors in data collection the system will expedite structural studies at higher resolution of less stable compounds and make possible analysis of larger macromolecules than we have previously studied.