PHILIPS CM10 TRANSMISSION ELECTRON MICROSCOPE

飞利浦 CM10 透射电子显微镜

• 批准号: 3519775
• 负责人: Thomas R Kozel
• 金额: $ 18.8万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-01-15 至 1988-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519775
• 关键词: cell cell interaction; electron microscopy; immunoelectron microscopy

The purpose of this grant is to obtain a general purpose Philips CM 10 transmission electron microscope. This is a "user friendly" instrument that is microprocessor controlled and is well-suited to the multiple needs of a diverse NIH-funded user group. The School of Medicine is a new developing institution that has experienced an explosion in the level of NIH research funding during the past five years. Maintaining this momentum is critically dependent on the acquisition of technology essential to existing and planned research activities. Our present microscope is a 16-year-old Hitachi 125-E. This is the only functioning TEM in both the School of Medicine and the University of Nevada. This instrument has been under service contract until 1985. A service contract is no longer possible because parts are not available or are difficult to locate. Thus, we are faced with the imminent loss of all TEM capability at the School of Medicine. This will have an immediate impact on existing NIH-funded projects that are critically dependent on TEM, and initiation of new directions that require TEM has become impossible. The current status of TEM facility has had an obvious impact on recruitment of new senior NIH-funded faculty who utilize TEM in their research The user group represents diverse research interests that include the ultrastructural location of opsonic proteins in microbial capsules (Kozel), electrophysiology and cell-to-cell coupling in the GI system (Rogers, Pubicover, and Sanders), molecular biology of Cytomegaloviruses (St Jeor), hormone-receptor interactions with intracellular proteins (Buxton), and the lethal mechanism of natural killer cells (Hudig). The specific applications of TEM are diverse, but the basic need for routine availability of TEM services is common to all users. As a consequence, the requested instrument will be a core facility designed to function as a service to the user group. The administration of the School of Medicine has commited the necessary resources to maintain a service contract on the instrument for its useable life and to employ a full time technician to maintain the instrument and provide TEM services for the NIH-funded user group.

该补助金的目的是获得一个通用飞利浦 CM 10透射电子显微镜。 这是一个“用户友好” 微处理器控制的仪器，非常适合于 不同NIH资助的用户群的多种需求。 医学院是一个新的发展机构， 经历了美国国立卫生研究院研究经费的爆炸式增长 在过去的五年里。 保持这一势头， 严重依赖于获得必要的技术， 现有和计划的研究活动。 我们现在的显微镜 是一台16年前的日立125-E 这是唯一能用的TEM 在医学院和内华达州大学。 这 该仪器的服务合同一直到1985年。 服务 合同不再可能，因为零件不可用或 很难找到 因此，我们面临着迫在眉睫的损失， 医学院所有TEM能力的一部分 这将有一个 对现有NIH资助项目的直接影响， 严格依赖TEM，并开始新的方向， 要求TEM已经变得不可能了。 TEM的现状 设施对招聘新的高级管理人员产生了明显的影响， NIH资助的在研究中使用TEM的教师 用户组代表了不同的研究兴趣，包括 微生物调理素蛋白超微结构定位 胶囊（Kozel），电生理学和细胞间耦合， 胃肠道系统（罗杰斯，Pubicover和桑德斯），分子生物学 巨细胞病毒（St Jeor），病毒-受体相互作用， 细胞内蛋白质（Buxton），以及 自然杀伤细胞（Hudig）。 TEM的具体应用有 多样化，但TEM常规可用性的基本需求 服务对所有用户都是通用的。 因此，所要求的 仪器将是一个核心设施，旨在作为一种服务 到用户组。 医学院的管理 已经投入了必要的资源来维持一项服务 合同的文书，其使用寿命和雇用 全职技术人员维护仪器并提供TEM 为NIH资助的用户群提供服务。