CULTURE PHYSIOLOGY AND ANTIGENIC ANALYSIS OF SEXUAL STAGES OF MALARIA PARASITES

本项目的目的是定义和分离编码P。 作为抗体阻断目标的恶性疟原虫抗原 这种寄生虫的配子体对蚊子的感染性。重头戏 针对恶性疟原虫有性期的单抗 已扩展为进一步定义目标抗原，其显示包括 面孔拟青霉230 kDa配子表面蛋白。克隆编码基因 因为这种抗原一直受到问题的困扰，包括 编码70 kDa热休克蛋白的DNA克隆的免疫选择 似乎在结构上与230 kDa配子表面蛋白有关 这就是搜索的目标。 现场研究表明，接触恶性疟原虫的人群 巴布亚新几内亚的传播，精心制作的230 kDa抗体 48 kDa和45 kDa配子表面蛋白。间日疟原虫的研究 斯里兰卡的研究表明，某些抗配子抗体可以抑制 在高浓度下寄生虫对蚊子的传染性，增强 在低浓度时具有传染性。具有特异性的人T细胞克隆 对于恶性疟原虫，配子抗原已经从日本人的血液中分离出来 没有免疫力的个体。这些克隆具有辅助性T细胞表型和 它们的反应性受到组织相容抗原的限制。这是有计划的 利用这些克隆来确定配子抗原的T细胞表位。 不同无性系间杂交试验分析。 恶性疟原虫正在几个实验室继续存在。杂交 已被证明会导致染色体大小的变化。

The object of this project is to define and isolate genes coding P. falciparum antigens which are the targets of antibodies which block infectivity of gametocytes of this parasite to mosquitoes. The repetoire of monoclonal antibodies specific to sexual stages of P. falciparum has been expanded to further define target antigens which are shown to include the 230 kDa gamete surface protein of P. faciparum. Cloning genes coding for such antigens has been beset with problems including the immunoselection of DNA clones coding a 70 kDa heat shock protein which appears to be structurally related to the 230 kDa gamete surface protein which was the object of the search. Field studies have shown that human populations exposed to P. falciparum transmission in Papua New Guinea, elaborate antibodies against the 230 kDa and the 48 kDa and 45 kDa gamete surface proteins. Studies with P. vivax in Sri Lanka have shown that certain antigamete antibodies which supress infectivity of the parasite to mosquitoes at high concentrations, enhance infectivity at low concentrations. Human T-cell clones with specificity for P. falciparum gamete antigens have been isolated from the blood of non-immune individuals. These clones have "helper" T-cell phenotypes and their reactivity is histocompatibility antigen restricted. It is planned to use such clones to define the T-cell epitopes of gamete antigens. Analysis of the cross hybridization experiment between clones of P. falciparum is being continued in several laboratories. Cross hybridization has been shown to lead to changes in chromosome size.