CULTURE PHYSIOLOGY AND ANTIGENIC ANALYSIS OF SEXUAL STAGES OF MALARIA PARASITES

抗配子传递阻断抗体靶抗原的研究 鸡疟原虫和恶性疟原虫形成一个由三种蛋白质组成的复合体 在疟原虫配子膜中，约有250、60和55kda。60岁的 55个KDA蛋白是疏水性的，可能包埋在膜上； 250 kDa的蛋白质是亲水性的。在鸡疫霉菌中，传播 阻断单抗只能识别恶性疟原虫单抗中的250 kDa蛋白 只识别60和55 KDA蛋白。目标表位似乎是 通过减少而被摧毁。 含恶性疟原虫DNA片段的Lambda GT-11重组克隆 从绿豆中分离出一种核酸酶消化酶。一个克隆可能包含 编码260 kDa蛋白质的DNA，另一种编码60和/或55 kda蛋白质的DNA； 第三，这样的克隆表达一种融合蛋白，该融合蛋白与 鸡疫霉卵动蛋白抗体。 分离One靶表位的特异性抗原变异 传播阻断单抗已在恶性疟原虫中得到确认。隔离 对不同按蚊物种的特定感染性差异 也得到了验证。恶性疟原虫不同的遗传克隆是 在遗传重组实验中进行杂交 通过蚊子和灵长类动物。 正在巴布亚新几内亚和#年进行疟疾传播研究。 斯里兰卡。斯里兰卡的研究表明，传播阻断 免疫是在间日疟原虫急性感染期间自然诱导的。

The target antigens of anti-gamete transmission blocking antibodies against P. gallinaceum and P. falciparum form a complex of three proteins of approximately 250, 60 and 55 Kda in the malarial gamete membrane. The 60 and 55 Kda proteins are hydrophobic and probably embedded in the membrane; the 250 Kda protein is hydrophilic. In P. gallinaceum the transmission blocking Mabs recognize only the 250 Kda protein in P. falciparum the Mabs recognize only the 60 and 55 Kda proteins. The target epitopes appears to be destroyed by reduction. Recombinant clones of Lambda gt-11 containing P. falciparum DNA fragments from a mung bean nuclease digest have been isolated. One clone may contain DNA coding the 260 Kda protein, another the 60 and/or 55 Kda protein; a third such clone expresses a fusion protein which cross reacts with antibodies against ookinetes of P. gallinaceum. Isolate specific antigenic variation for the target epitope of one transmission blocking Mab has been confirmed in P. falciparum. Isolate specific differences in infectivity to different Anopheline species have also been demonstrated. Genetically distinct clones of P. falciparum are being hybridized in genetic recombination experiments during transmission through mosquitoes and primates. Malaria transmission studies are being conducted in Papua New Guinea and in Sri Lanka. The studies in Sri Lanka have shown that transmission blocking immunity is naturally induced during acute infections of P. vivax.