DIFFERENTIATION OF LEISHMANIA PROMASTIGOTES

利什曼原虫前鞭毛体的分化

• 批准号: 3960504
• 负责人: D L SACKS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960504
• 关键词: India; Leishmania; antimicroorganism antibody; complement pathway; host organism interaction; immune tolerance /unresponsiveness; immunotherapy; interleukin 2; leishmaniasis; macrophage; microorganism culture; microorganism growth; monoclonal antibody; protozoal antigen; suppressor T lymphocyte; virulence

Metacyclic promastigotes are highly virulent to a susceptible vertebrate host. This virulence appears to be related to the ability of metacyclic forms to resist killing by the alternative complement pathway, and to their ability to resist the microbicidal activities of normal resident macrophages. A monoclonal antibody has been raised which recognizes a cell surface and secreted molecule which is unique to metacyclic promastigotes. It appears to be the exclusive molecule actively secreted by infective stage organisms. It is, therefore, likely to play a biologically critical role in promoting successful parasitism. Its chemistry and mode of action are being pursued. Approximately 2 months of each year are spent studying the immunology of visceral leishmaniasis in north India. Patients with active disease are specifically unresponsive at the T cell level to leishmanial antigens. This unresponsiveness cannot be reversed by depletion of T8 "suppressor" cells or by reconstitution with IL-2. We have recently detected a potentially large population of exposed, asymptomatic individuals from endemic areas based on their reactivity to parasite antigens in vivo and in vitro. The nature of the antigens recognized by the "immune" T cells is under study.

代谢环前鞭毛体对易感脊椎动物具有高度毒性 主持人 这种毒力似乎与代谢环的能力有关。 形式，以抵抗通过替代补体途径的杀伤，以及它们的 抵抗正常居民的杀微生物活动的能力 巨噬细胞 一种单克隆抗体已经研制成功， 它是后环前鞭毛体特有的表面和分泌分子。 它似乎是感染性病毒主动分泌的唯一分子。 阶段生物。 因此，它很可能在生物学上起到关键作用， 促进成功寄生的作用。 它的化学成分和作用方式 正在被追捕 每年大约有2个月的时间用于研究 印度北部的内脏利什曼病。 活动性疾病患者 在T细胞水平对利什曼原虫抗原特异性无应答。 这种无反应性不能通过耗尽T8“抑制因子”来逆转。 细胞或用IL-2重建。 我们最近发现了一个 潜在的大量暴露人群，无症状的个人， 流行地区的基础上，他们的反应，寄生虫抗原在体内和 体外 由“免疫”T细胞识别的抗原的性质是 正在研究中。