DIFFERENTIATION OF LEISHMANIA PROMASTIGOTES

利什曼原虫前鞭毛体的分化

• 批准号: 3822012
• 负责人: D L SACKS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822012
• 关键词: India; Leishmania; antimicroorganism antibody; complement pathway; glycolipids; host organism interaction; immune tolerance /unresponsiveness; immunotherapy; interleukin 2; leishmaniasis; macrophage; microorganism culture; microorganism growth; monoclonal antibody; protozoal antigen; sialate; suppressor T lymphocyte; virulence

The differentiation of Leishmania promastigotes from a non- infective to an infective or metacyclic stage is accompanied by changes in surface carbohydrates as detected by the lectin PNA. This change is a consequence of the sialation of a cell surface and released glycolipid, which can be identified by the monoclonal antibody 3F12, which is specific for metacyclic promastigotes of L. major. The expression of the modified form of the glycolipid appears to influence the type of receptors on the macrophage which the organisms use the attachment and uptake. Metacyclic promastigotes can be shown to use CR1 receptors, because their attachment can be inhibited by mAb against these receptors. Non-infective promastigotes from log phase cultures cannot be shown to utilize C3 receptors. Opsonization of promastigotes with C3 enhances their uptake and promotes their survival, indicating that the choice of receptors for attachment influences subsequent intracellular survival. The molecular nature of these receptor ligand interactions and consequences will be the basis of further studies in terms of the role of the glycolipid and in particular the sialic acid, the role of other cellular receptors for carbohydrates, and the role of complement activation and C3b deposition in these events.

利什曼原虫前鞭毛体与非前鞭毛体的鉴别 感染到感染或代谢循环阶段， 通过凝集素PNA检测的表面碳水化合物的变化。 这种变化是细胞表面唾液酸化的结果， 释放的糖脂，可以通过单克隆抗体 抗体3F12，其特异性针对 L.少校 糖脂修饰形式的表达 似乎会影响巨噬细胞上受体的类型 生物体利用它来附着和吸收。 亚循环 前鞭毛体可以被证明使用CR1受体，因为它们 抗这些受体的单克隆抗体可抑制粘附。 来自对数期培养物的非感染性前鞭毛体不能 显示利用C3受体。 前鞭毛体的调理作用 与C3增强他们的吸收和促进他们的生存， 这表明，对依恋受体的选择会影响 随后的细胞内存活。 这些受体配体相互作用的分子性质和 结果将是进一步研究的基础， 糖脂，特别是唾液酸的作用， 碳水化合物的其他细胞受体，以及 补体激活和C3b沉积。