STRUCTURE AND FUNCTION OF THE ADV GENOME

这个项目的目的是研究基因组的结构和功能 阿留申病的水貂细小病毒（ADV）。 大约85%的 减毒ADV-G株的DNA序列已使用 基于M13的双脱氧方法。 研究结果表明， 组织类似于其他无缺陷的细小病毒，但 在核苷酸和预测的蛋白质水平上的同源性较低， 超过50%。 代表15-88个图谱单位（MU）的分子克隆， 直接来源于两种体内传代的强毒ADV毒株， 与ADV-G相比。 详细的限制性酶切图谱显示， 是非常密切相关的，所有三种病毒的片段都是 同样的尺寸。 在E.大肠杆菌， 与病毒体、衣壳蛋白特异性血清反应，但是，尽管 DNA片段长度相同，蛋白质 两种病毒的克隆编码的蛋白质分子量均比对照组大2-3 kD， 关于ADV-G 这一发现可能很重要，因为发现的衣壳蛋白 在来自强毒病毒的颗粒中，也比来自弱毒病毒的颗粒大2-3 kD。 可比较的ADV-G衣壳蛋白。 DNA序列比较 两种病毒和ADV-G正在进行中。

The purpose of this project is the study of genome structure and function of the Aleutian disease of mink parvovirus (ADV). Approximately 85% of the DNA sequence of the attenuated ADV-G strain has been deduced using the M13-based dideoxy methods. The findings suggest that the overall organization is similar to that of other nondefective parvoviurses, but that homology at both the nucleotide and predicted protein level is less than 50%. Molecular clones representing the 15-88 map units (MU) were derived directly from two in vivo passaged virulent ADV strains and compared to ADV-G. Detailed restriction mapping indicated that the viruses were very closely related and that the segments of all three viruses were the same size. Clones of all three expressed antigens in E. coli that reacted with sera specific for virion, capsid proteins, but, in spite of the fact that the DNA segments are identical in length, the proteins encoded by clones of the two in vivo viruses were 2-3 kd larger than those of ADV-G. This finding may be important because the capsid proteins found in particles from virulent viruses also are 2-3 kd larger than the comparable ADV-G capsid proteins. DNA sequence comparison between these two viruses and ADV-G is underway.