DNA DAMAGE BY ALKYLATING AGENTS AND THEIR REPAIR IN HUMAN TUMOR CELLS

已有研究表明，大约20%的人类肿瘤株和病毒 转化的线条对烷基化试剂高度敏感，这是因为 明显缺乏烷基鸟嘌呤烷基转移酶。我们已经能够 从人的肝脏中部分提纯酶。这种酶能去除 鸟嘌呤06位上的烷基化损伤，而在其他位置上未见 DNA我们计划进一步鉴定这种酶，并将其提纯到 可以获得氨基酸序列信息的点。在……里面 与D.Yarosh的结合，人类肝脏提取物正被用于 分离出足够数量的这种蛋白质。中美之间的差异 Mer+细胞和Mer-细胞中特定RNA的转录也是 正在接受调查。

It has been shown that approximately 20% of human tumor lines and viral transformed lines are hypersensitive to alkylating agents due to an apparent absence of Alkylguanine Alkyltransferase. We have been able to partially purify the enzyme from human liver. This enzyme removes alkylation damage at the 06 position of guanine but not at other sites in DNA. We plan to further characterize this enzyme and purify it to the point where amino acid sequence information can be obtained. In collaberation with D. Yarosh, human liver extracts are being used to isolate adequate quantities of this protein. Differences in the transcription of specific RNA's in mer plus and mer minus cells are also being investigated.