RAS P21 PROTEINS AND INDENTIFYING DBL ONCOGENE PRODUCT

项目1：在我们不断努力了解结构-功能的过程中 与rasp 21的关系，我们进行了4-5个氨基酸的框内缺失 在p21蛋白区域作为抗ras p21单克隆抗体的表位 抗体，Y13-259。 这种抗体可以逆转p21的转化作用， 并且还可以阻断DNA合成的起始。 Y13-259 不直接干扰p21的已知生化特性。 这些 研究表明，这种抗体可能会影响一些尚未发现的 p21的功能 深入了解Y13-259结合的功能 p21区域，目前正在分析这些突变体的 生物化学和生物学特性。 项目2：从人弥漫性B细胞中分离的一种新癌基因db 1 淋巴瘤，以前已经显示出赋予转化表型， NIH/3 T3细胞。 我们目前的研究是为了 鉴定db 1癌基因产物，并表征其某些 结构和功能特性。 一种66，000道尔顿的蛋白质（p66） 在已获得db 1癌基因的细胞中鉴定，而在未获得db 1癌基因的细胞中鉴定， 存在于由其他癌基因转化的细胞或未转化的NIH/3 T3中 细胞 此外，确定p66由db 1编码 癌基因 p66是一种磷蛋白，丝氨酸是其主要的磷酸化位点。 磷酸化，它是一种细胞质蛋白， 亚细胞分布

Project 1: In our continued effort to understand the structure-function relationship of ras p21, we have made inframe deletions of 4-5 amino acids in the region of p21 protein as the epitope for anti-ras p21 monoclonal antibody, Y13-259. This antibody can revert the transforming effect of p21 and can also block the initiation of DNA synthesis. However, Y13-259 does not directly interfere with known biochemical properties of p21. These studies suggest that this antibody may affect some as yet undetected function of p21. To gain insights into the function of Y13-259 binding region of p21, these mutants are currently being analyzed for their biochemical and biological properties. Project 2: A novel oncogene, db1, isolated from a human diffuse B-cell lymphoma, has been previously shown to confer the transformed phenotype on NIH/3T3 cells. Our present studies were undertaken in an effort to identify the db1 oncogene product and to characterize some of its structural and functional properties. A 66,000 dalton protein (p66) has been identified in cells that have acquired db1 oncogene, while it is not present in cells transformed by other oncogenes or in untransformed NIH/3T3 cells. Furthermore, it is established that p66 is encoded by db1 oncogene. p66 is a phosphoprotein with serine a major site of phosphorylation and it is a cytoplasmic protein as judged by its subcellular distribution.