GENETIC AND MOLECULAR ANALYSIS OF THE CUT LOCUS OF D MELANOGASTER

黑腹果蝇切割位点的遗传和分子分析

• 批准号: 3965278
• 负责人: J W JACK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3965278
• 关键词: DNA; alleles; cell differentiation; chromosome deletion; gene expression; gene mutation; genetic mapping; genetic regulation; genetic transcription; molecular cloning; mutant; nucleic acid sequence; virus genetics

We are interested in knowing how cells of a single organism can differentiate to form specific tissue types. We have chosen to address one aspect of the question by learning how one gene, the cut locus of Drosophila melanogaster, is expressed differently in different tissues of the fly. We have now cloned DNA sequences representing the entire gene, which encompasses 200 kb or more of DNA. A large number of mutants have been analyzed. We find that the deletion of sequences in the leftmost part of the gene cause phenotypic effects primarily in the legs, while deletion of or insertions into sequences slightly to the right cause effects primarily in the wings. Mutations in a 70 kb to the right affect the wings, head, and thorax and cause lethality. A fourth group of mutations lacks cut locus function in all tissues. These mutations map at the rightmost end of the gene. The availability of tissue specific mutants of a gene afford the opportunity to experiment to find out how the gene normally operates in tissue specific ways. We are currently studying the transcriptional activity of the cut locus to find out how the tissue specificity of the mutant phenotypes relates to the transcriptional activity of the gene. We now know that many of the cut mutants are insertions of retrovirus-like sequences into the cut locus DNA, and we are interested in understanding the affect of these sequences on gene activity. Some of these mutations are suppressible and will be useful in determining how a mutation caused by a retrovirus-like sequence can be suppressed.

我们感兴趣的是了解单个有机体的细胞如何 分化形成特定的组织类型。 我们选择了解决一个 问题的一个方面，通过学习如何一个基因，切割位点， 在果蝇的不同组织中表达不同， 苍蝇 我们现在已经克隆了代表整个基因的DNA序列， 包括200 kb或更多的DNA。 大量的突变体已经被 分析了 我们发现，在最左边的部分序列的缺失， 该基因主要在腿部引起表型效应，而缺失 或插入到稍微靠右的序列中， 在机翼上。 右边70 kb的突变会影响翅膀，头部， 造成致命伤 第四组突变缺乏切割 所有组织中的基因座功能。 这些突变映射在 基因 基因的组织特异性突变体的可用性提供了 有机会进行实验，以找出基因在正常情况下是如何运作的。 组织特异性方式。 我们目前正在研究 活性的切割位点，以找出如何组织特异性的切割位点， 突变表型与基因的转录活性有关。 我们现在知道，许多切割突变体是逆转录病毒样的插入， 插入到切割位点DNA中，我们有兴趣了解 这些序列对基因活性的影响。 这些突变中的一些 是可抑制的，这将有助于确定由以下因素引起的突变是如何发生的： 可以抑制逆转录病毒样序列。