DETERMINATION OF AMINES AND AMINE METABOLITES IN BIOLOGICAL SAMPLES

生物样品中胺和胺代谢物的测定

• 批准号: 3964199
• 负责人: K L KIRK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3964199
• 关键词: acylation; amines; analytical method; blood chemistry; cerebrospinal fluid; clinical chemistry; electrochemistry; high performance liquid chromatography; neurotransmitter metabolism; neurotransmitters; plasma; urinalysis

The importance of biogenic amines in neurotransmission, metabolism, and in control of various physiological processes has spurred intense interest in the development of precise and sensitive methods for the quantitation of these amines and their metabolites present in cerebrospinal fluid, plasma and urine. We have developed precise, operationally simple procedures for the analyses of certain of these substances based on HPLC separation using highly sensitive electrochemical detection, coupled with the use of internal standards for accurate quantitation. The utility of the approach has been extended greatly by selective and efficient acylation of the endogenous amines which permits ready isolation from the biological sample. Use of an electroactive acylating reagent further extends this approach to the accurate detection of amines which, themselves, are not amenable to electrochemical detection (e.g., histamine and phenethylamine).

生物胺在神经传递、代谢和免疫调节中的重要性 对各种生理过程的控制引起了人们对 开发精确和灵敏的方法， 这些胺及其代谢产物存在于脑脊液、血浆 和尿液。 我们开发了精确的、操作简单的分析程序 基于HPLC分离的某些这些物质， 灵敏的电化学检测，加上使用内部 准确定量的标准。 该方法的实用性已被 通过选择性和有效的酰化内源性 胺，其允许容易地从生物样品中分离。 用途 电活性酰化试剂进一步将这种方法扩展到 准确检测胺，其本身不适合于 电化学检测（例如，组胺和苯乙胺）。