HISTAMINE RELEASE FROM BEIGE MOUSE MAST CELLS

我们正在继续研究米色小鼠的肥大细胞。 人Chediak-Higashi综合征模型。我们研究这个细胞是因为 其非常大的分泌颗粒，就像 中性粒细胞处于患病状态。免疫抑制的一个缺陷是 综合征是指分泌功能不全。米色老鼠桅杆的大尺寸 细胞有可能详细分析它们对血浆的胞吐作用 薄膜。去年，我们开发了一种仪器阵列，能够 同时进行生理学和解剖学实时生命测量 控制内部毫米数的细胞，使用它来测量 小鼠腹膜分泌细胞和吞噬细胞的电容 米色小鼠体内灌流的肥大细胞。因此，我们可以结合 生物化学、解剖学和生理学通过对 单个有源单元同时记录两种光学信息(图像， 胞吐、内吞、收缩)和电信息(离子 电流、电压钳位、电容)，分辨率为17毫秒。初步 研究表明，分泌颗粒的融合先于 小泡肿胀17-150毫秒。已经过的颗粒 被高渗溶液收缩后也能很好地与细胞膜融合。 在检测到肿胀之前。显然，在这个系统中，囊泡肿胀是 膜融合不需要，但可能需要释放 分泌性产品。

We are continuing to study the mast cell of the beige mouse, an animal model for the Chediak-Higashi syndrome in man. We study this cell because of its very large secretory granules, like the large granules of the neutrophils in the diseased state. One defect in that immuno-suppressed syndrome is failure in secretion. The large size of the beige mouse mast cell make possible detailed analysis of their exocytosis to the plasma membrane. Last year, we developed an instrumental array capable of simultaneous physiologic and anatomic real-time measurements of living cells with control of the internal millieu, using it to measure the capacitance of secretory and phagocytic peritoneal cells from the internally perfused mast cell from the beige mouse. Thus, we can combine biochemistry, anatomy, and physiology by performing perfusion studies on single active cells while recording both optical information (image, exocytosis, endocytosis, contraction), and electrical information (ionic currents, voltage clamp, capacitance) with 17 msec resoultion. Preliminary studies reveal that the fusion of the secretory granule preceeds the swelling of that vesicle by 17-150 milliseconds. Granules which have been shrunken by hyperosmotic solutions also fuse to the cell membrane well before detectable swelling. Apparently in this system vesicle swelling is not needed for membrane fusion but may be required for the release of secretory products.