HISTAMINE RELEASE FROM BEIGE MOUSE MAST CELLS

米色小鼠是Chediak-Higashi综合征的动物模型， 伙计 这种动物肥大细胞的大颗粒使得 详细分析了它们对质膜的胞吐作用。 我们有 开发了一种仪器阵列，能够同时进行生理和 活细胞的解剖学实时测量， 内毫微区，首次用它来测量分泌细胞的电容， 和吞噬性腹膜细胞。 我们设计了一种 纯化肥大细胞的方法。 大电容显示较大电容 虽然巨噬细胞比肥大细胞小 这 悖论表明，在巨噬细胞膜折叠的高度， 这一发现与其在吞噬作用中的作用一致。 大电容 在肥大细胞融合的瞬间观察到变化，与 分泌颗粒的计算面积。 个体肿胀 还可以看到颗粒。 预计同时测量 肿胀和融合将证明或反驳胞吐作用的渗透理论。

The beige mouse is an animal model for the Chediak-Higashi syndrome in man. The large granules of the mast cell of that animal make possible detailed analysis of their exocytosis to the plasma membrane. We have developed an instrumental array capable of simultaneous physiologic and anatomic real-time measurements of living cells with control of the internal millieu, first using it to measure the capacitance of secretory and phagocytic peritoneal cells from the beige mouse. We have devised a method for purification of mast cells. Macrophages show larger capacitance than the mast cell, although the macrophage is a smaller cell. This paradox suggests a high degree of membrane folding in the macrophage, a finding consistent with its role in phagocytosis. Large capacitance changes are seen at the instant of fusion in mast cells, consistent with the calculated area of the secretory granule. Swelling of individual granules is also seen. It is expected that simultaneous measurement of swelling and fusion will prove or disprove the osmotic theory of exocytosis.