THE ROLE OF GROWTH HORMONE IN THE AGING PROCESS

我们的假设是生长激素缺乏可能是某些 与衰老有关的生理变化，包括矿物质从 骨骼，降低皮肤弹性和瘦体重的损失。 该项目的具体目标是：(1)确定S的年龄 生长抑素C(Sm-C)和平均积分显著下降 血清生长激素水平(ICGH)在正常男性和 女性。(2)确定男性年龄是否大于50岁且年龄较低 血浆中ICGH和Sm-C水平在给药时从垂体释放GH 引起儿童和年轻人生长激素释放的药理刺激。 (3)确定与氮气和钙有关的急性代谢反应 平衡，脂和脂蛋白浓度，糖耐量， 当给予生长激素时，羟脯氨酸排泄量和Sm-C浓度 正常男性(>45岁)5-7天，GH和Sm-C低 水平，与生长激素缺乏的年轻人的反应相比较。 (4)确定每日服用生长激素3年对小鼠的影响。 生长激素缺乏者20-30岁(HA组)和 45岁以上正常男性(组)体成分的研究 o)。HA和O组将分为两组(HA1和HA2组10组，HA2组15组 01和02)。01&HA1将每天接受生长激素治疗。02和HA2将作为 控制。评估的身体成分参数包括全身 氮、钙、脂肪、瘦体重、骨密度、全身水分和皮肤 组成。穿孔活检的皮肤将被分析出类型和 用十二烷基硫酸钠电泳法测定I、III型胶原蛋白含量； 免疫荧光抗体技术检测层粘连蛋白和IV型胶原； AA法测定羟脯氨酸和羟赖氨酸水平：可还原交叉法 用层析法测定胶原蛋白中的连接.纤维束直径的测量 和电子显微镜定位；以及血浆纤维连接蛋白水平。 所有身体成分的研究将在不同的个人之间进行比较 在组内和组间确定随年龄和年龄的变化 长期服用生长激素引起的改变。 除了获得的科学知识外，可以想象的是 生长激素可以使身体组织恢复到更早的状态 老年人的管理。

Our hypothesis is that GH deficiency may be responsible for some physiologic changes associated with aging, including loss of mineral from bone, decreased skin elasticity and loss of lean body mass. The specific aims of the project are: (1) To establish the age(s) at which a significant decrease in somatomedin-C (Sm-C) and mean integrated concentrations of serum GH levels (ICGH) occurs in normal males and females. (2) To determine if males Greater than 50 years of age with low ICGH & Sm-C levels in plasma release GH from the pituitary when given pharmacologic stimuli which cause GH release in children & young adults. (3) To determine the acute metabolic responses relating to N2 and Ca balance, lipid and lipoprotein concentrations, glucose tolerance, hydroxyproline excretion, and to Sm-C concentrations, when GH is given for 5-7 days to normal males (Greater than 45 years), with low GH and Sm-C levels, as compared to the response of young adults who are GH deficient. (4) To establish the effect of daily GH administration for 3 years on the body composition of GH deficient subjects 20-30 years of age (Group HA) and on the body composition of normal males Greater than 45 years of age (Group O). Groups HA and O will be divided into 2 groups (10 in HA1 & HA2, 15 in 01 & 02). 01 & HA1 will receive GH each day. 02 & HA2 will serve as controls. Parameters of body composition to be assessed include total body N2, Ca, fat, lean body mass, bone density, total body water, and skin composition. Skin from punch biopsies will be analyzed for the type and quantity of Type I & III collagen by SDS electrophoresis; fibronectin, laminin, and Type IV collagen by immunofluorescent antibody techniques; levels of hydroxyproline and hydroxylysine by AA analysis: reducible cross links in collagen by chromatography; measurement of fiber bundle diameter and orientation by electron microscopy; and fibronectin levels in plasma. Comparison of all body composition studies will be made between individuals in groups and between groups to ascertain changes with aging and alterations as a result of long term GH administration. In addition to the scientific knowledge gained, it is conceivable that restoration of body tissues to an earlier state could occur with GH administration in older individuals.