ETHANOL EFFECTS ON CNS TISSUE IN VIVO AND IN VITRO

乙醇暴露对哺乳动物中枢神经系统的影响 将使用细胞外和细胞内 在原位完整脑、体外脑切片和 眼内脑移植 研究将集中在遗传变异 表现出不同的酒精相关行为作为模型系统， 检查急性乙醇作用以及研究耐受性， 对酒精的依赖 本研究项目的长期目标 有三种。 首先，电生理反应的机制， 乙醇将被表征为直接的、调节的或涉及局部的 回路中间神经元 第二，对乙醇的反应的变化，以及 神经递质系统的功能变化将在 诱导耐受或依赖。 最后，遗传变异将 允许乙醇诱导的电生理反应与 酒精引起的行为。 更深入地了解乙醇如何改变 中枢神经系统功能应该为酒精问题提供新的见解 中毒和酗酒。

The effects of ethanol exposure on mammalian central nervous system function will be pursued using both extracellular and intracellular recording in the intact brain in situ, in the in vitro brain slice and in intraocular brain transplants. Studies will focus on genetic variants manifesting differential alcohol-related behaviors as model systems for examining acute ethanol actions as well as for studying tolerance and dependence to alcohol. The long-term objectives of this research program are three fold. First, the mechanism of electrophysiological responses to ethanol will be characterized as direct, modulatory or involving local circuit interneurons. Second, changes in responses to ethanol, as well as functional changes in neurotransmitter systems will be determined after induction of tolerance or dependence. Finally, the genetic variants will permit ethanol-induced electrophysiological responses to be correlated with alcohol-induced behaviors. A greater understanding of how ethanol alters CNS function should furnish new insights into the problems of alcohol intoxication and alcoholism.