TRIPLE LASER-MULTI PARAMETER FLOW CYTOMETRY FOR STUDY OF TUMOR CELL KINETICS

用于肿瘤细胞动力学研究的三重激光多参数流式细胞术

• 批准号: 3978290
• 负责人: W H SCHUETTE
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3978290
• 关键词: Trypanosoma cruzi; cellular oncology; computers; cytology; flow cytometry; fluorescent dye /probe; human tissue; immunofluorescence technique; neoplasm /cancer immunodiagnosis; neoplastic cell; radiotracer

A Triple laser flow cytometer has been developed so that various immuno-fluorescent labeling techniques may be employed for the investigation of cell kinetics. Three laser beams at different wave lengths are made to intersect a tumor cell flow stream passing through a quartz cuvette so that multi-parameter signals may be obtained. These signals are processed by specialized electronics and then analyzed by means of a PDP 11 computer. Simplified optics have increased light detection efficiency by an order of magnitude. The system is currently being used for the investigation of a unicellular protozoon known as trypanosoma cruzi, the causative agent of Chagas disease. Electronic cell volume capability has been added to the system to assist in the bivariate analysis of trypanosoma cruzi fluorescence data.

一种三激光流式细胞仪已经被开发出来，以使各种 免疫荧光标记技术可用于 细胞动力学研究。三束不同波长的激光 长度被设置为与流经 石英试管，可获得多参数信号。这些 信号由专门的电子器件处理，然后通过 一台PDP 11计算机。简化的光学系统提高了光探测能力 效率提高了一个数量级。系统当前正在使用中 用于研究一种被称为锥虫的单细胞原虫 克鲁兹，恰加斯病的病原体。电子细胞体积 系统中已添加了帮助进行双变量分析的功能 克氏锥虫的荧光数据。