TRIPLE LASER-MULTI PARAMETER FLOW CYTOMETRY FOR STUDY OF TUMOR CELL KINETICS

用于肿瘤细胞动力学研究的三重激光多参数流式细胞术

• 批准号: 3959929
• 负责人: W H SCHUETTE
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3959929
• 关键词: Candida; bacterial genetics; cellular oncology; computer simulation; cytology; flow cytometry; human tissue; immunofluorescence technique; radiotracer

A triple-laser flow cytometer has been developed so that various immuno-fluorescent labeling techniques may be employed for the investigation of cell kinetics. The electronics portion of the system has been redesigned to be compatible with an IBM AT personal computer. The new design was facilitated by first developing an analog flow-cytometry simulator, complete with computer hand shaking. This simulator was used extensively in the development of the new software for the system. Gating, ratio subtraction and logarithmic preprocessing are all done by the computer, greatly reducing the analog electronic pre- processing requirements. The high optical sensitivity of the basic system has permitted the flow cytometric analysis of the DNA synthetic cycle of Candida Species.

三激光流式细胞仪已经开发，使得各种 免疫荧光标记技术可用于 细胞动力学研究。 的电子部分 系统已重新设计，与IBM AT兼容 个人电脑 新的设计是由第一个 开发模拟流式细胞术模拟器， 电脑手抖 该模拟器被广泛用于 新系统软件的开发。 门控， 比率减法和对数预处理都是通过 计算机，大大减少了模拟电子预处理， 加工要求。 基本的高光学灵敏度 系统允许对DNA进行流式细胞仪分析 假丝酵母属的合成周期。