REGULATION OF GENE EXPRESSION AND DIFFERENTIATION IN NEOPLASIA

这个项目的目的是研究基因表达的调控 在两个实验模型系统中与细胞分化相关; 1)人早幼粒细胞白血病细胞系HL 60，和2）大鼠 肝癌细胞系，Escherber和7777。 在HL 60中获得的结果 细胞系研究包括c-myc RNA水平与 细胞的分化状态。 具体地，c-myc的表达 几乎完全与HL 60的早幼粒细胞状态相关 这种表达在细胞分化过程中受到抑制， 早幼粒细胞到单核细胞。 相反，N-ras特异性转录是 在分化特异性或生长过程中， 具体治疗。 取得的成果和正在进行的实验， 大鼠肝癌细胞研究包括白蛋白特异性基因应答 随后用丁酸或ADP-核糖基化抑制剂处理。 这种反应与细胞的“分化状态”无关， 通过甲胎蛋白（AFP）或白蛋白的产生来测量， 白蛋白特异性反应存在于高白蛋白、高AFP 生产细胞系（AFPber）或低白蛋白、低AFP生产细胞系 （7777）。 AP基因在两组中均未受到该治疗方案的影响。 细胞系

The object of this project is to examine the regulation of gene expression associated with cellular differentiation in two experimental model systems; 1) the human promyelocytic leukemia cell line, HL60, and 2) the rat hepatoma cell lines, Reuber and 7777. The results obtained in the HL60 cell line study include a functional assocaition of c-myc RNA levels with the differentiation state of the cell. Specifically, expression of c-myc is almost exclusively associated with the promyelocytic status of the HL60 cells and this expression is repressed during differentiation of the promyelocytes to monocytes. Conversely N-ras specific transcription is eff-ted to no significant extent during differentiation specific or growth specific treatment. The results obtained and experiments in progress in the rat hepatoma cell study include an albumin specific gene response following treatment with butyric acid or inhibitors of ADP-ribosylation. This response was independent of the "differentiation state" of the cells, as measured by alpha-fetoprotein (AFP) or albumin production, since the albumin specific response was present in the high albumin, high AFP producer cell line (Reuber) or the low albumin, low AFP producer line (7777). The AP gene was not affected by this treatment protocol in either cell line.