MOLECULAR CLONING OF GENES ASSOCIATED WITH CHEMICAL HEPATOCARCINOGENESIS

该项目的目的是分离和鉴定基因， 与大鼠肝脏系统中的肿瘤转化相关。 这 该项目将与实验室的其他成员联合进行 他们已经分离出或正在分离蛋白质， 肿瘤状态的特征。 为此，我们有（1） 在γ-谷氨酰转肽酶II中构建cDNA文库， 正常和肿瘤大鼠肝脏，（2）构建基因组文库， Fischer大鼠基因组的EMBL载体系统，和（3）正在进行的过程中， 亚克隆和测序先前发现的相关的cDNA克隆 老鼠肝脏的再生 我们还分离了一个基因组克隆， 广泛使用的肝肿瘤标志物γ-谷氨酰转肽酶和基因 目前正在对该克隆进行表达研究。

The objective of this project is to isolate and identify genes which are associated with neoplastic transformation in the rat liver system. This project will be in join affiliation with other members of the laboratory who have isolated, or are in the process of isolating, proteins which are characteristic of the neoplastic state. To this end we have (1) constructed cDNA libraries in gamma-glutamyl transpeptidase II to both normal and neoplastic rat liver, (2) constructed a genomic library to the Fischer rat genome using EMBL vector system, and (3) are in the process of subcloning and sequencing a cDNA clone previously found to be associated with regenerating rat liver. We have also isolated a genomic clone for the widely used liver tumor marker, gamma-glutamyl transpeptidase, and gene expression studies with this clone are in progress.